Chapter title |
Structural basis of photoswitching in fluorescent proteins.
|
---|---|
Chapter number | 12 |
Book title |
Photoswitching Proteins
|
Published in |
Methods in molecular biology, March 2014
|
DOI | 10.1007/978-1-4939-0470-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0469-3, 978-1-4939-0470-9
|
Authors |
Chenxi Duan, Virgile Adam, Martin Byrdin, Dominique Bourgeois, Duan, Chenxi, Adam, Virgile, Byrdin, Martin, Bourgeois, Dominique |
Editors |
Sidney Cambridge |
Abstract |
Fluorescent proteins have revolutionized life sciences because they allow noninvasive and highly specific labeling of biological samples. The subset of "phototransformable" fluorescent proteins recently attracted a widespread interest, as their fluorescence state can be modified upon excitation at defined wavelengths. The fluorescence emission of Reversibly Switchable Fluorescent Proteins (RSFPs), in particular, can be repeatedly switched on and off. RSFPs enable many new exciting modalities in fluorescence microscopy and biotechnology, including protein tracking, photochromic Förster Resonance Energy Transfer, super-resolution microscopy, optogenetics, and ultra-high-density optical data storage. Photoswitching in RSFPs typically results from chromophore cis-trans isomerization accompanied by a protonation change, but other switching schemes based on, e.g., chromophore hydration/dehydration have also been discovered. In this chapter, we review the main structural features at the basis of photoswitching in RSFPs. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Hungary | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 6% |
France | 1 | 3% |
Unknown | 28 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 9 | 29% |
Student > Ph. D. Student | 7 | 23% |
Student > Master | 4 | 13% |
Student > Bachelor | 2 | 6% |
Professor | 2 | 6% |
Other | 3 | 10% |
Unknown | 4 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 26% |
Agricultural and Biological Sciences | 7 | 23% |
Chemistry | 4 | 13% |
Medicine and Dentistry | 2 | 6% |
Physics and Astronomy | 2 | 6% |
Other | 3 | 10% |
Unknown | 5 | 16% |