Title |
Maghemite, silver, ceragenin conjugate particles for selective binding and contrast of bacteria
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Published in |
Journal of Colloid & Interface Science, September 2013
|
DOI | 10.1016/j.jcis.2013.09.016 |
Pubmed ID | |
Authors |
Mark A. Hoppens, Zaanan E.W. Wheeler, Ammar T. Qureshi, Katie Hogan, Ashleigh Wright, George G. Stanley, David Young, Paul Savage, Daniel Hayes |
Abstract |
New synthesis techniques are providing increasing control over many inorganic nanoparticle characteristics, facilitating the creation of new multifunctional theranostics. This report proposes the synthesis and testing of a combination nanoparticle comprised of a maghemite core for enhanced T2 MRI contrast diagnostics, a colloidal silver shell acting as an antimicrobial and therapeutic vehicle, and a ceragenin (CSA-124) surfactant providing microbial adhesion. A polyacrylic acid functionalized maghemite nanoparticle is synthesized by a high temperature organic phase reduction followed by thiol functionalization and gold cluster seeding. A silver shell is formed through AgNO3 reduction, and an oriented monolayer of the thiolated ceragenin, is bound through a self-assembly process. The process and products are characterized throughout synthesis through TEM, DLS, FT-IR, UV-Vis, ICP-OES, HPLC-ESI-TOF-MS, DC magnetization and susceptibility, X-ray diffraction, and in vitro MRI. Synthesized Diagnostic Antimicrobial Nanoparticles (DANs) were found to have a spherical morphology with a diameter of 32.47±1.83 nm, hydrodynamic diameter of 53.05±1.20 nm, maximum magnetic moment of 12 emu/g NP (54 emu/g Fe) with little variation due to temperature, and are predominantly paramagnetic. In vitro MRI studies show that DANs contrast well at concentrations as low as 9 ppm, and successfully adhere to Staphylococcus aureus. DAN MIC was determined to be approximately 12 ppm and 24 ppm against S. aureus and Escherichia coli respectively. |
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Geographical breakdown
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Unknown | 23 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 32% |
Researcher | 3 | 12% |
Student > Master | 3 | 12% |
Professor > Associate Professor | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 4 | 16% |
Unknown | 3 | 12% |
Readers by discipline | Count | As % |
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Materials Science | 3 | 12% |
Agricultural and Biological Sciences | 3 | 12% |
Immunology and Microbiology | 2 | 8% |
Physics and Astronomy | 2 | 8% |
Other | 6 | 24% |
Unknown | 5 | 20% |