Title |
Chiral Resolution and Pharmacological Characterization of the Enantiomers of the Hsp90 Inhibitor 2‐Amino‐7‐[4‐fluoro‐2‐(3‐pyridyl)phenyl]‐4‐methyl‐7,8‐dihydro‐6H‐quinazolin‐5‐one Oxime
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Published in |
ChemMedChem, April 2014
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DOI | 10.1002/cmdc.201400037 |
Pubmed ID | |
Authors |
Raffaella Amici, Chiara Bigogno, Roberto Boggio, Andrea Colombo, Stephen M. Courtney, Roberto Dal Zuffo, Giulio Dondio, Fulvia Fusar, Stefania Gagliardi, Saverio Minucci, Marco Molteni, Christian A. G. N. Montalbetti, Annalisa Mortoni, Mario Varasi, Stefania Vultaggio, Ciro Mercurio |
Abstract |
Heat-shock protein 90 (Hsp90) is a molecular chaperone involved in the stabilization of key oncogenic signaling proteins, and therefore, inhibition of Hsp90 represents a new strategy in cancer therapy. 2-Amino-7-[4-fluoro-2-(3-pyridyl)phenyl]-4-methyl-7,8-dihydro-6H-quinazolin-5-one oxime is a racemic Hsp90 inhibitor that targets the N-terminal adenosine triphosphatase site. We developed a method to resolve the enantiomers and evaluated their inhibitory activity on Hsp90 and the consequent antitumor effects. The (S) stereoisomer emerged as a potent Hsp90 inhibitor in biochemical and cellular assays. In addition, this enantiomer exhibited high oral bioavailability in mice and excellent antitumor activity in two different human cancer xenograft models. |
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