| Title |
An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology
|
|---|---|
| Published in |
Brain Structure and Function, April 2014
|
| DOI | 10.1007/s00429-014-0730-z |
| Pubmed ID | |
| Authors |
Matthias Georg Haberl, Silvia Viana da Silva, Jason M. Guest, Melanie Ginger, Alexander Ghanem, Christophe Mulle, Marcel Oberlaender, Karl-Klaus Conzelmann, Andreas Frick |
| Abstract |
Glycoprotein-deleted rabies virus (RABV ∆G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV ∆G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV ∆G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (>14 months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV ∆G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 145 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 3% |
| Netherlands | 2 | 1% |
| United Kingdom | 2 | 1% |
| Switzerland | 1 | <1% |
| France | 1 | <1% |
| Unknown | 135 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 42 | 29% |
| Student > Ph. D. Student | 29 | 20% |
| Student > Master | 21 | 14% |
| Student > Bachelor | 9 | 6% |
| Professor > Associate Professor | 7 | 5% |
| Other | 19 | 13% |
| Unknown | 18 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 46 | 32% |
| Agricultural and Biological Sciences | 42 | 29% |
| Medicine and Dentistry | 13 | 9% |
| Biochemistry, Genetics and Molecular Biology | 6 | 4% |
| Veterinary Science and Veterinary Medicine | 4 | 3% |
| Other | 13 | 9% |
| Unknown | 21 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2021.
All research outputs
#6,540,389
of 24,217,893 outputs
Outputs from Brain Structure and Function
#473
of 1,725 outputs
Outputs of similar age
#59,369
of 231,263 outputs
Outputs of similar age from Brain Structure and Function
#10
of 29 outputs
Altmetric has tracked 24,217,893 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 1,725 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,263 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.