| Title |
Characterization of hybrid cells derived from spontaneous fusion events between breast epithelial cells exhibiting stem-like characteristics and breast cancer cells
|
|---|---|
| Published in |
Clinical & Experimental Metastasis, October 2010
|
| DOI | 10.1007/s10585-010-9359-3 |
| Pubmed ID | |
| Authors |
Thomas Dittmar, Sarah Schwitalla, Jeanette Seidel, Sonja Haverkampf, Georg Reith, Sönke Meyer-Staeckling, Burkhard H. Brandt, Bernd Niggemann, Kurt S. Zänker |
| Abstract |
Several data of the past years clearly indicated that the fusion of tumor cells and tumor cells or tumor cells and normal cells can give rise to hybrids cells exhibited novel properties such as an increased malignancy, drug resistance, or resistance to apoptosis. In the present study we characterized hybrid cells derived from spontaneous fusion events between the breast epithelial cell line M13SV1-EGFP-Neo and two breast cancer cell lines: HS578T-Hyg and MDA-MB-435-Hyg. Short-tandem-repeat analysis revealed an overlap of parental alleles in all hybrid cells indicating that hybrid cells originated from real cell fusion events. RealTime-PCR-array gene expression data provided evidence that each hybrid cell clone exhibited a unique gene expression pattern, resulting in a specific resistance of hybrid clones towards chemotherapeutic drugs, such as doxorubicin and paclitaxel, as well as a specific migratory behavior of hybrid clones towards EGF. For instance, M13MDA435-4 hybrids showed a marked resistance towards etoposide, doxorubicin and paclitaxel, whereas hybrid clones M13MDA-435-1 and -2 were only resistant towards etoposide. Likewise, all investigated M13MDA435 hybrids responded to EGF with an increased migratory activity, whereas the migration of parental MDA-MB-435-Hyg cells was blocked by EGF, suggesting that M13MDA435 hybrids may have acquired a new motility pathway. Similar findings have been obtained for M13HS hybrids. We conclude from our data that they further support the hypothesis that cell fusion could give rise to drug resistant and migratory active tumor (hybrid) cells in cancer. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 3% |
| Unknown | 30 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 26% |
| Student > Master | 4 | 13% |
| Student > Doctoral Student | 3 | 10% |
| Student > Bachelor | 2 | 6% |
| Researcher | 2 | 6% |
| Other | 7 | 23% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 29% |
| Agricultural and Biological Sciences | 8 | 26% |
| Medicine and Dentistry | 5 | 16% |
| Mathematics | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 6 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 April 2018.
All research outputs
#7,917,073
of 23,854,458 outputs
Outputs from Clinical & Experimental Metastasis
#210
of 778 outputs
Outputs of similar age
#36,826
of 101,863 outputs
Outputs of similar age from Clinical & Experimental Metastasis
#2
of 6 outputs
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