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Re-classification within the serogroups O3 and O8 of Citrobacter strains

Overview of attention for article published in BMC Microbiology, July 2017
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Title
Re-classification within the serogroups O3 and O8 of Citrobacter strains
Published in
BMC Microbiology, July 2017
DOI 10.1186/s12866-017-1078-3
Pubmed ID
Authors

Ewa Katzenellenbogen, Magdalena Staniszewska, Nina A. Kocharova, Małgorzata Mieszała, Agnieszka Korzeniowska-Kowal, Sabina Górska, Yuriy A. Knirel, Andrzej Gamian

Abstract

Citrobacter strains are opportunistic pathogens often responsible for serious enteric as well as extra-intestinal diseases, and therefore the O-antigenic scheme, still in use in diagnostic identification, should be set for proper serotyping. The structures of more than 30 different Citrobacter O-antigens (O-polysaccharide chains of the lipopolysaccharides) of 43 Citrobacter O-serogroups have been elucidated so far. However, relationships between strains in several heterogeneous serogroups still need to be clarified by immunochemical studies. These include complex serogroups O3 and O8, represented by 20 and 7 strains, respectively, which are the subject of the present work. Earlier, the O-polysaccharide structures have been determined for Citrobacter O3 strain Be35/57 (PCM 1508) and Citrobacter O8 strain Be64/57 (PCM 1536). Serological studies (immunoblotting) carried out on Citrobacter lipopolysaccharides from different strains ascribed to serogroups O3 and O8 showed that each of these serogroups should be divided into non-cross-reacting subgroups. Based on the results of chemical analyses and (1)H and (13)C NMR spectroscopy the structure of Citrobacter O-antigens from strains PCM 1504 (O6) and PCM 1573 (O2) have been established. Chemical data combined with serological analyses showed that several Citrobacter strains should be reclassified into other serogroups. Immunochemical studies carried out on Citrobacter LPS, described in this paper, showed the expediency of reclassification of: 1) strains PCM 1504 and PCM 1573 from serogroups O6 and O2 to serogroups O3 and O8, respectively, 2) strains PCM 1503 and PCM 1505 from serogroups O3 and O8 to new serogroups O3a and O8a, respectively.

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Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 17%
Researcher 2 17%
Lecturer 1 8%
Professor 1 8%
Student > Doctoral Student 1 8%
Other 2 17%
Unknown 3 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 17%
Veterinary Science and Veterinary Medicine 1 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Nursing and Health Professions 1 8%
Biochemistry, Genetics and Molecular Biology 1 8%
Other 2 17%
Unknown 4 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2017.
All research outputs
#20,440,241
of 22,994,508 outputs
Outputs from BMC Microbiology
#2,701
of 3,207 outputs
Outputs of similar age
#277,018
of 317,332 outputs
Outputs of similar age from BMC Microbiology
#41
of 52 outputs
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