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Solution NMR structure of the TRIM21 B-box2 and identification of residues involved in its interaction with the RING domain

Overview of attention for article published in PLOS ONE, July 2017
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Title
Solution NMR structure of the TRIM21 B-box2 and identification of residues involved in its interaction with the RING domain
Published in
PLOS ONE, July 2017
DOI 10.1371/journal.pone.0181551
Pubmed ID
Authors

Amélie Wallenhammar, Madhanagopal Anandapadamanaban, Alexander Lemak, Claudio Mirabello, Patrik Lundström, Björn Wallner, Maria Sunnerhagen

Abstract

Tripartite motif-containing (TRIM) proteins are defined by the sequential arrangement of RING, B-box and coiled-coil domains (RBCC), where the B-box domain is a unique feature of the TRIM protein family. TRIM21 is an E3 ubiquitin-protein ligase implicated in innate immune signaling by acting as an autoantigen and by modifying interferon regulatory factors. Here we report the three-dimensional solution structure of the TRIM21 B-box2 domain by nuclear magnetic resonance (NMR) spectroscopy. The structure of the B-box2 domain, comprising TRIM21 residues 86-130, consists of a short α-helical segment with an N-terminal short β-strand and two anti-parallel β-strands jointly found the core, and adopts a RING-like fold. This ββαβ core largely defines the overall fold of the TRIM21 B-box2 and the coordination of one Zn2+ ion stabilizes the tertiary structure of the protein. Using NMR titration experiments, we have identified an exposed interaction surface, a novel interaction patch where the B-box2 is likely to bind the N-terminal RING domain. Our structure together with comparisons with other TRIM B-box domains jointly reveal how its different surfaces are employed for various modular interactions, and provides extended understanding of how this domain relates to flanking domains in TRIM proteins.

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Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 29%
Student > Ph. D. Student 6 25%
Student > Master 3 13%
Student > Bachelor 2 8%
Student > Postgraduate 2 8%
Other 1 4%
Unknown 3 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 54%
Agricultural and Biological Sciences 4 17%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Chemical Engineering 1 4%
Chemistry 1 4%
Other 0 0%
Unknown 4 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 July 2017.
All research outputs
#20,440,241
of 22,994,508 outputs
Outputs from PLOS ONE
#175,120
of 196,028 outputs
Outputs of similar age
#276,354
of 316,684 outputs
Outputs of similar age from PLOS ONE
#3,690
of 4,086 outputs
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