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Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells

Overview of attention for article published in Nature, April 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

news
28 news outlets
blogs
6 blogs
twitter
127 X users
patent
4 patents
weibo
10 weibo users
facebook
5 Facebook pages
wikipedia
3 Wikipedia pages

Citations

dimensions_citation
179 Dimensions

Readers on

mendeley
301 Mendeley
citeulike
4 CiteULike
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Title
Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells
Published in
Nature, April 2014
DOI 10.1038/nature13287
Pubmed ID
Authors

Mitsutoshi Yamada, Bjarki Johannesson, Ido Sagi, Lisa Cole Burnett, Daniel H. Kort, Robert W. Prosser, Daniel Paull, Michael W. Nestor, Matthew Freeby, Ellen Greenberg, Robin S. Goland, Rudolph L. Leibel, Susan L. Solomon, Nissim Benvenisty, Mark V. Sauer, Dieter Egli

Abstract

The transfer of somatic cell nuclei into oocytes can give rise to pluripotent stem cells that are consistently equivalent to embryonic stem cells, holding promise for autologous cell replacement therapy. Although methods to induce pluripotent stem cells from somatic cells by transcription factors are widely used in basic research, numerous differences between induced pluripotent stem cells and embryonic stem cells have been reported, potentially affecting their clinical use. Because of the therapeutic potential of diploid embryonic stem-cell lines derived from adult cells of diseased human subjects, we have systematically investigated the parameters affecting efficiency of blastocyst development and stem-cell derivation. Here we show that improvements to the oocyte activation protocol, including the use of both kinase and translation inhibitors, and cell culture in the presence of histone deacetylase inhibitors, promote development to the blastocyst stage. Developmental efficiency varied between oocyte donors, and was inversely related to the number of days of hormonal stimulation required for oocyte maturation, whereas the daily dose of gonadotropin or the total number of metaphase II oocytes retrieved did not affect developmental outcome. Because the use of concentrated Sendai virus for cell fusion induced an increase in intracellular calcium concentration, causing premature oocyte activation, we used diluted Sendai virus in calcium-free medium. Using this modified nuclear transfer protocol, we derived diploid pluripotent stem-cell lines from somatic cells of a newborn and, for the first time, an adult, a female with type 1 diabetes.

X Demographics

X Demographics

The data shown below were collected from the profiles of 127 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 301 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 2%
United Kingdom 3 <1%
Iran, Islamic Republic of 2 <1%
France 2 <1%
Sweden 1 <1%
Turkey 1 <1%
Belgium 1 <1%
Mexico 1 <1%
Spain 1 <1%
Other 1 <1%
Unknown 283 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 56 19%
Student > Ph. D. Student 53 18%
Student > Bachelor 43 14%
Student > Master 26 9%
Other 23 8%
Other 63 21%
Unknown 37 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 108 36%
Biochemistry, Genetics and Molecular Biology 73 24%
Medicine and Dentistry 44 15%
Immunology and Microbiology 6 2%
Neuroscience 5 2%
Other 25 8%
Unknown 40 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 361. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2023.
All research outputs
#87,810
of 25,353,525 outputs
Outputs from Nature
#6,263
of 97,360 outputs
Outputs of similar age
#645
of 234,745 outputs
Outputs of similar age from Nature
#58
of 1,004 outputs
Altmetric has tracked 25,353,525 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 97,360 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 102.4. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 234,745 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,004 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.