| Title |
Oxidative damage repair by glutamine in fish enterocytes
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, April 2014
|
| DOI | 10.1007/s10695-014-9938-3 |
| Pubmed ID | |
| Authors |
Kai Hu, Lin Feng, Weidan Jiang, Yang Liu, Jun Jiang, Shuhong Li, Xiaoqiu Zhou |
| Abstract |
Fish intestine is very sensitive to oxidative damage. Repair of damaged enterocytes may be involved to restore normal function of fish intestine. However, studies of fish enterocyte repair are scarce. The present study aimed to investigate the potential repair role of glutamine after a H2O2 challenge. In this study, fish enterocytes were post-treated with graded levels of glutamine (0, 4, 8, 12 and 20 mM of glutamine) after expose to 100 μM H2O2. The basal control cells were kept in the glutamine-free minimum essential medium only. Results showed that the H2O2-induced decreases in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide optical density, alkaline phosphatase and Na(+), K(+)-ATPase activities were completely restored by subsequent glutamine treatments. In addition, cellular injury (lactate dehydrogenase), lipid peroxidation (malondialdehyde) and protein oxidation (protein carbonyls) caused by H2O2 were reversed by subsequent glutamine treatments. Furthermore, the H2O2-induced decreases in glutathione contents, glutathione reductase, superoxide dismutase and glutathione peroxidase activities were completely restored by subsequent glutamine treatments. In summary, the present study indicated that glutamine improved the repair activity in fish enterocytes after challenge with H2O2. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 19% |
| Student > Master | 3 | 14% |
| Lecturer | 2 | 10% |
| Other | 2 | 10% |
| Professor | 2 | 10% |
| Other | 7 | 33% |
| Unknown | 1 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 48% |
| Medicine and Dentistry | 3 | 14% |
| Veterinary Science and Veterinary Medicine | 2 | 10% |
| Environmental Science | 2 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 2 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2014.
All research outputs
#18,371,293
of 22,754,104 outputs
Outputs from Fish Physiology and Biochemistry
#404
of 859 outputs
Outputs of similar age
#164,501
of 227,503 outputs
Outputs of similar age from Fish Physiology and Biochemistry
#4
of 8 outputs
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