Title |
Mycoplasma pneumoniae and/or Chlamydophila pneumoniae inoculation causing different aggravations in cholesterol-induced atherosclerosis in apoE KO male mice
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Published in |
BMC Microbiology, September 2009
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DOI | 10.1186/1471-2180-9-194 |
Pubmed ID | |
Authors |
Sueli B Damy, Maria L Higuchi, Jorge Timenetsky, Márcia M Reis, Suely P Palomino, Renata N Ikegami, Fabiana P Santos, Junko T Osaka, Luiz P Figueiredo |
Abstract |
Chamydophila pneumoniae (CP) and/or Mycoplasma pneumoniae (MP) are two bacteria detected in vulnerable atheromas. In this study we aimed to analyze whether CP and/or MP aggravates atherosclerosis induced by cholesterol-enriched diet in C57BL/6 apoE KO male mice. Thirty male apoE KO mice aged eight weeks fed by a diet containing 1% cholesterol until 32 weeks of age were divided into four groups: the first was inoculated with CP (n = 7), the second with MP (n = 12), the third with both CP + MP (n = 5), and the fourth with saline (sham n = 6). The animals were re-inoculated at 36 weeks of age, and sacrificed at 40 weeks of age. Two ascending aorta and one aortic arch segments were sampled. In the most severely obstructed segment, vessel diameter, plaque height, percentage of luminal obstruction and the degree of adventitial inflammation were analyzed. The plaque area/intimal surface ratio was obtained by measuring all three segments. The adventitial inflammation was semiquantified (0 absent, 1 mild, 2 moderate, and 3 diffuse). |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 7% |
Netherlands | 1 | 7% |
Unknown | 13 | 87% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 20% |
Student > Ph. D. Student | 3 | 20% |
Professor > Associate Professor | 2 | 13% |
Student > Master | 2 | 13% |
Unspecified | 1 | 7% |
Other | 3 | 20% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 6 | 40% |
Agricultural and Biological Sciences | 3 | 20% |
Unspecified | 1 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 13% |