| Title |
Sox9 gene regulation and the loss of the XY/XX sex-determining mechanism in the mole vole Ellobius lutescens
|
|---|---|
| Published in |
Chromosome Research, January 2012
|
| DOI | 10.1007/s10577-011-9269-5 |
| Pubmed ID | |
| Authors |
Stefan Bagheri-Fam, Rajini Sreenivasan, Pascal Bernard, Kevin C. Knower, Ryohei Sekido, Robin Lovell-Badge, Walter Just, Vincent R. Harley |
| Abstract |
In most mammals, the Y chromosomal Sry gene initiates testis formation within the bipotential gonad, resulting in male development. SRY is a transcription factor and together with SF1 it directly up-regulates the expression of the pivotal sex-determining gene Sox9 via a 1.3-kb cis-regulatory element (TESCO) which contains an evolutionarily conserved region (ECR) of 180 bp. Remarkably, several rodent species appear to determine sex in the absence of Sry and a Y chromosome, including the mole voles Ellobius lutescens and Ellobius tancrei, whereas Ellobius fuscocapillus of the same genus retained Sry. The sex-determining mechanisms in the Sry-negative species remain elusive. We have cloned and sequenced 1.1 kb of E. lutescens TESCO which shares 75% sequence identity with mouse TESCO indicating that testicular Sox9 expression in E. lutescens might still be regulated via TESCO. We have also cloned and sequenced the ECRs of E. tancrei and E. fuscocapillus. While the three Ellobius ECRs are highly similar (94-97% sequence identity), they all display a 14-bp deletion (Δ14) removing a highly conserved SOX/TCF site. Introducing Δ14 into mouse TESCO increased both basal activity and SF1-mediated activation of TESCO in HEK293T cells. We propose a model whereby Δ14 may have triggered up-regulation of Sox9 in XX gonads leading to destabilization of the XY/XX sex-determining mechanism in Ellobius. E. lutescens/E. tancrei and E. fuscocapillus could have independently stabilized their sex determination mechanisms by Sry-independent and Sry-dependent approaches, respectively. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 2% |
| Unknown | 49 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 26% |
| Researcher | 13 | 26% |
| Student > Master | 6 | 12% |
| Student > Bachelor | 5 | 10% |
| Student > Doctoral Student | 2 | 4% |
| Other | 0 | 0% |
| Unknown | 11 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 40% |
| Biochemistry, Genetics and Molecular Biology | 14 | 28% |
| Psychology | 2 | 4% |
| Nursing and Health Professions | 1 | 2% |
| Environmental Science | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 10 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2024.
All research outputs
#3,561,001
of 25,126,845 outputs
Outputs from Chromosome Research
#48
of 537 outputs
Outputs of similar age
#27,966
of 256,228 outputs
Outputs of similar age from Chromosome Research
#3
of 12 outputs
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