| Title |
Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, August 2011
|
| DOI | 10.1007/s00253-011-3492-z |
| Pubmed ID | |
| Authors |
Xiongjun Shao, Babu Raman, Mingjun Zhu, Jonathan R. Mielenz, Steven D. Brown, Adam M. Guss, Lee R. Lynd |
| Abstract |
Clostridium thermocellum is a model microorganism for converting cellulosic biomass into fuels and chemicals via consolidated bioprocessing. One of the challenges for industrial application of this organism is its low ethanol tolerance, typically 1-2% (w/v) in wild-type strains. In this study, we report the development and characterization of mutant C. thermocellum strains that can grow in the presence of high ethanol concentrations. Starting from a single colony, wild-type C. thermocellum ATCC 27405 was sub-cultured and adapted for growth in up to 50 g/L ethanol using either cellobiose or crystalline cellulose as the growth substrate. Both the adapted strains retained their ability to grow on either substrate and displayed a higher growth rate and biomass yield than the wild-type strain in the absence of ethanol. With added ethanol in the media, the mutant strains displayed an inverse correlation between ethanol concentration and growth rate or biomass yield. Genome sequencing revealed six common mutations in the two ethanol-tolerant strains including an alcohol dehydrogenase gene and genes involved in arginine/pyrimidine biosynthetic pathway. The potential role of these mutations in ethanol tolerance phenotype is discussed. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Nepal | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 1% |
| Unknown | 68 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 19 | 28% |
| Student > Ph. D. Student | 15 | 22% |
| Student > Bachelor | 6 | 9% |
| Student > Master | 6 | 9% |
| Student > Doctoral Student | 3 | 4% |
| Other | 7 | 10% |
| Unknown | 13 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 43% |
| Biochemistry, Genetics and Molecular Biology | 14 | 20% |
| Engineering | 3 | 4% |
| Chemical Engineering | 2 | 3% |
| Immunology and Microbiology | 2 | 3% |
| Other | 3 | 4% |
| Unknown | 15 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2020.
All research outputs
#6,908,521
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,404
of 8,034 outputs
Outputs of similar age
#37,581
of 126,670 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#22
of 58 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 69% of its peers.
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We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.