| Title |
OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity
|
|---|---|
| Published in |
Transgenic Research, May 2014
|
| DOI | 10.1007/s11248-014-9803-2 |
| Pubmed ID | |
| Authors |
Jiexue Huang, Maohong Cai, Qizhang Long, Linglong Liu, Qiuyun Lin, Ling Jiang, Saihua Chen, Jianmin Wan |
| Abstract |
Rice production and seed storage are confronted with grain deterioration and loss of seed viability. Some members of the lipoxygenase (LOX) family function in degradation of storage lipids during the seed germination, but little is known about their influence on seed longevity during storage. We characterized the role of rice OsLOX2 gene in seed germination and longevity via over-expression and knock-down approaches. Abundant expression of OsLOX2 was detected in panicles, roots, and stems, but not in leaves. Moreover, OsLOX2 was highly induced during germination. OsLOX2 protein, located in the cytoplasm, showed a wide range of temperature adaptation (20-50 °C) and a substrate preference to linoleic acid. Lines over-expressing OsLOX2 showed accelerated seed germination under normal condition and lower seed viability after accelerated aging. RNA interference (RNAi) of OsLOX2 caused delayed germination and enhanced seed longevity. RNAi lines with strongly repressed OsLOX2 activity completely lost the capability of germination after accelerated aging. More lipid hydroperoxide were found in OE15 than the control, but less in RNAi lines than in the WT Nipponbare. Therefore, OsLOX2 acts in opposite directions during seed germination and longevity during storage. Appropriate repression of the OsLOX2 gene may delay the aging process during the storage without compromising germination under normal conditions. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 49 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 20% |
| Student > Master | 8 | 16% |
| Researcher | 8 | 16% |
| Lecturer | 3 | 6% |
| Other | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 15 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 57% |
| Biochemistry, Genetics and Molecular Biology | 6 | 12% |
| Unspecified | 1 | 2% |
| Unknown | 15 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2015.
All research outputs
#15,300,431
of 22,755,127 outputs
Outputs from Transgenic Research
#715
of 891 outputs
Outputs of similar age
#133,953
of 227,581 outputs
Outputs of similar age from Transgenic Research
#5
of 8 outputs
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