| Title |
Metastatic breast cancer cells colonize and degrade three-dimensional osteoblastic tissue in vitro
|
|---|---|
| Published in |
Clinical & Experimental Metastasis, June 2008
|
| DOI | 10.1007/s10585-008-9185-z |
| Pubmed ID | |
| Authors |
Ravi Dhurjati, Venkatesh Krishnan, Laurie A. Shuman, Andrea M. Mastro, Erwin A. Vogler |
| Abstract |
Metastatic breast cancer cells (BCs) colonize a mineralized three-dimensional (3D) osteoblastic tissue (OT) grown from isolated pre-osteoblasts for up to 5 months in a specialized bioreactor. Sequential stages of BC interaction with OT include BC adhesion, penetration, colony formation, and OT reorganization into "Indian files" paralleling BC colonies, heretofore observed only in authentic pathological cancer tissue. BCs permeabilize OT by degrading the extra-cellular collagenous matrix (ECM) in which the osteoblasts are embedded. OT maturity (characterized by culture age and cell phenotype) profoundly affects the patterns of BC colonization. BCs rapidly form colonies on immature OT (higher cell/ECM ratio, osteoblastic phenotype) but fail to completely penetrate OT. By contrast, BCs efficiently penetrate mature OT (lower cell/ECM ratio, osteocytic phenotype) and reorganize OT. BC colonization provokes a strong osteoblast inflammatory response marked by increased expression of the pro-inflammatory cytokine IL-6. Furthermore, BCs inhibit osteoblastic bone formation by down-regulating synthesis of collagen and osteocalcin. Results strongly suggest that breast cancer disrupts the process of osteoblastic bone formation, in addition to upregulating osteoclastic bone resorption as widely reported. These observations may help explain why administration of bisphosphonates to humans with osteolytic metastases slows lesion progression by inhibiting osteoclasts but does not bring about osteoblast-mediated healing. |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 2 | 3% |
| United States | 1 | 2% |
| Portugal | 1 | 2% |
| Switzerland | 1 | 2% |
| Unknown | 56 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 25% |
| Student > Bachelor | 10 | 16% |
| Researcher | 9 | 15% |
| Student > Master | 8 | 13% |
| Student > Doctoral Student | 5 | 8% |
| Other | 5 | 8% |
| Unknown | 9 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 28% |
| Engineering | 12 | 20% |
| Medicine and Dentistry | 8 | 13% |
| Biochemistry, Genetics and Molecular Biology | 6 | 10% |
| Mathematics | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 15 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 April 2009.
All research outputs
#16,171,492
of 23,854,458 outputs
Outputs from Clinical & Experimental Metastasis
#533
of 778 outputs
Outputs of similar age
#71,140
of 83,714 outputs
Outputs of similar age from Clinical & Experimental Metastasis
#11
of 11 outputs
Altmetric has tracked 23,854,458 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 778 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 83,714 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.