| Title |
Single-Cell Transcriptome Analysis of Developing and Regenerating Spiral Ganglion Neurons
|
|---|---|
| Published in |
Current Pharmacology Reports, August 2016
|
| DOI | 10.1007/s40495-016-0064-z |
| Pubmed ID | |
| Authors |
Kelvin Y. Kwan |
| Abstract |
The spiral ganglion neurons (SGNs) of the cochlea are essential for our ability to hear. SGN loss after exposure to ototoxic drugs or loud noise results in hearing loss. Pluripotent stem cell-derived and endogenous progenitor cell types have the potential to become SGNs and are cellular foundations for replacement therapies. Repurposing transcriptional regulatory networks to promote SGN differentiation from progenitor cells is a strategy for regeneration. Advances in the Fludigm C1 workflow or Drop-seq allow sequencing of single cell transcriptomes to reveal variability between cells. During differentiation, the individual transcriptomes obtained from single-cell RNA-seq can be exploited to identify different cellular states. Pseudotemporal ordering of transcriptomes describes the differentiation trajectory, allows monitoring of transcriptional changes and determines molecular barriers that prevent the progression of progenitors into SGNs. Analysis of single cell transcriptomes will help develop novel strategies for guiding efficient SGN regeneration. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 1 | 25% |
| France | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 20% |
| Student > Ph. D. Student | 5 | 17% |
| Student > Bachelor | 4 | 13% |
| Professor | 3 | 10% |
| Student > Doctoral Student | 1 | 3% |
| Other | 4 | 13% |
| Unknown | 7 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 7 | 23% |
| Medicine and Dentistry | 5 | 17% |
| Engineering | 3 | 10% |
| Biochemistry, Genetics and Molecular Biology | 2 | 7% |
| Immunology and Microbiology | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 10 | 33% |
Attention Score in Context
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#15,851,587
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#1
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Outputs of similar age from Current Pharmacology Reports
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