| Title |
Cryopreservation of human ovarian tissue using the silver closed vitrification system
|
|---|---|
| Published in |
Journal of Assisted Reproduction and Genetics, July 2017
|
| DOI | 10.1007/s10815-017-1004-5 |
| Pubmed ID | |
| Authors |
Zhun Xiao, Yaoyao Zhang, Wei Fan |
| Abstract |
The aim of this study is to evaluate the feasibility of using a hand-made silver container for the cryopreservation of human ovarian cortex. Human ovarian cortex tissues were vitrified using an open vitrification system (OVS) of needle immersed vitrification (NIV) and two closed vitrification systems (CVS) of a plastic vial (plastic CVS) and a silver container (silver CVS). Outcomes of vitrification were evaluated morphologically and histologically by in vitro culture and xenotransplantation. The apoptosis of primordial follicles was assessed by TUNEL staining. The production of E2 and P4 was examined by a chemiluminescent immunoassay. Blood vessels were visualized with CD31 staining. Compared with the fresh ovarian cortex tissue, ovarian cortex tissues that were vitrified using the three different carriers and then warmed showed significantly reduced percentages of normal primordial follicles, viability of primordial follicles, E2 and P4 levels during in vitro culture and decreased amounts of blood vessels. However, much better outcomes were obtained with NIV and silver CVS than with plastic CVS, based on the better morphology and viability of primordial follicles, higher E2 and P4 production during an in vitro culture, and greater numbers of blood vessels after xenografting. Importantly, the outcomes of ovarian cortex cryopreservation with silver CVS were similar and comparable to those with NIV. The hand-made silver container as a CVS is a promising carrier for the cryopreservation of the human ovarian cortex. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 17% |
| Student > Bachelor | 4 | 13% |
| Student > Master | 3 | 10% |
| Researcher | 3 | 10% |
| Student > Doctoral Student | 2 | 7% |
| Other | 7 | 23% |
| Unknown | 6 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 20% |
| Medicine and Dentistry | 6 | 20% |
| Agricultural and Biological Sciences | 5 | 17% |
| Materials Science | 2 | 7% |
| Nursing and Health Professions | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 8 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 August 2017.
All research outputs
#21,608,038
of 24,119,703 outputs
Outputs from Journal of Assisted Reproduction and Genetics
#1,433
of 1,697 outputs
Outputs of similar age
#280,699
of 320,026 outputs
Outputs of similar age from Journal of Assisted Reproduction and Genetics
#27
of 30 outputs
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