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Inducible somatic embryogenesis in Theobroma cacao achieved using the DEX-activatable transcription factor-glucocorticoid receptor fusion

Overview of attention for article published in Biotechnology Techniques, July 2017
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Title
Inducible somatic embryogenesis in Theobroma cacao achieved using the DEX-activatable transcription factor-glucocorticoid receptor fusion
Published in
Biotechnology Techniques, July 2017
DOI 10.1007/s10529-017-2404-4
Pubmed ID
Authors

Morgan E. Shires, Sergio L. Florez, Tina S. Lai, Wayne R. Curtis

Abstract

To carry out mass propagation of superior plants to improve agricultural and silvicultural production though advancements in plant cell totipotency, or the ability of differentiated somatic plant cells to regenerate an entire plant. The first demonstration of a titratable control over somatic embryo formation in a commercially relevant plant, Theobroma cacao (Chocolate tree), was achieved using a dexamethasone activatable chimeric transcription factor. This four-fold enhancement in embryo production rate utilized a glucocorticoid receptor fused to an embryogenic transcription factor LEAFY COTYLEDON 2. Where previous T. cacao somatic embryogenesis has been restricted to dissected flower parts, this construct confers an unprecedented embryogenic potential to leaves. Activatable chimeric transcription factors provide a means for elucidating the regulatory cascade associated with plant somatic embryogenesis towards improving its use for somatic regeneration of transgenics and plant propagation.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 42 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 16%
Researcher 7 16%
Student > Master 5 12%
Student > Bachelor 4 9%
Professor 2 5%
Other 4 9%
Unknown 14 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 47%
Biochemistry, Genetics and Molecular Biology 5 12%
Nursing and Health Professions 1 2%
Chemical Engineering 1 2%
Economics, Econometrics and Finance 1 2%
Other 1 2%
Unknown 14 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2017.
All research outputs
#20,881,763
of 25,655,374 outputs
Outputs from Biotechnology Techniques
#2,360
of 2,768 outputs
Outputs of similar age
#253,744
of 327,736 outputs
Outputs of similar age from Biotechnology Techniques
#13
of 25 outputs
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So far Altmetric has tracked 2,768 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
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