| Title |
Properties, production, and applications of camelid single-domain antibody fragments
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, August 2007
|
| DOI | 10.1007/s00253-007-1142-2 |
| Pubmed ID | |
| Authors |
M. M. Harmsen, H. J. De Haard |
| Abstract |
Camelids produce functional antibodies devoid of light chains of which the single N-terminal domain is fully capable of antigen binding. These single-domain antibody fragments (VHHs or Nanobodies) have several advantages for biotechnological applications. They are well expressed in microorganisms and have a high stability and solubility. Furthermore, they are well suited for construction of larger molecules and selection systems such as phage, yeast, or ribosome display. This minireview offers an overview of (1) their properties as compared to conventional antibodies, (2) their production in microorganisms, with a focus on yeasts, and (3) their therapeutic applications. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,213 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 10 | <1% |
| Germany | 3 | <1% |
| Mexico | 3 | <1% |
| United Kingdom | 3 | <1% |
| Belgium | 2 | <1% |
| Netherlands | 2 | <1% |
| Norway | 1 | <1% |
| Brazil | 1 | <1% |
| Canada | 1 | <1% |
| Other | 9 | <1% |
| Unknown | 1178 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 233 | 19% |
| Student > Master | 219 | 18% |
| Researcher | 200 | 16% |
| Student > Bachelor | 188 | 15% |
| Student > Doctoral Student | 51 | 4% |
| Other | 118 | 10% |
| Unknown | 204 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 349 | 29% |
| Biochemistry, Genetics and Molecular Biology | 307 | 25% |
| Chemistry | 72 | 6% |
| Immunology and Microbiology | 58 | 5% |
| Medicine and Dentistry | 56 | 5% |
| Other | 138 | 11% |
| Unknown | 233 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 57. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2024.
All research outputs
#744,636
of 25,323,244 outputs
Outputs from Applied Microbiology and Biotechnology
#23
of 8,273 outputs
Outputs of similar age
#1,073
of 75,254 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#3
of 68 outputs
Altmetric has tracked 25,323,244 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,273 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 75,254 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.