| Title |
Characteristic CSF Prion Seeding Efficiency in Humans with Prion Diseases
|
|---|---|
| Published in |
Molecular Neurobiology, May 2014
|
| DOI | 10.1007/s12035-014-8709-6 |
| Pubmed ID | |
| Authors |
Maria Cramm, Matthias Schmitz, André Karch, Saima Zafar, Daniela Varges, Eva Mitrova, Bjoern Schroeder, Alex Raeber, Franziska Kuhn, Inga Zerr |
| Abstract |
The development of in vitro amplification systems allows detecting femtomolar amounts of prion protein scrapie (PrP(Sc)) in human cerebrospinal fluid (CSF). We performed a CSF study to determine the effects of prion disease type, codon 129 genotype, PrP(Sc) type, and other disease-related factors on the real-time quaking-induced conversion (RT-QuIC) response. We analyzed times to 10,000 relative fluorescence units, areas under the curve and the signal maximum of RT-QuIC response as seeding parameters of interest. Interestingly, type of prion disease (sporadic vs. genetic) and the PRNP mutation (E200K vs. V210I and FFI), codon 129 genotype, and PrP(Sc) type affected RT-QuIC response. In genetic forms, type of mutation showed the strongest effect on the observed outcome variables. In sporadic CJD, MM1 patients displayed a higher RT-QuIC signal maximum compared to MV1 and VV1. Age and gender were not associated with RT-QuIC signal, but patients with a short disease course showed a higher seeding efficiency of the RT-QuIC response. This study demonstrated that PrP(Sc) characteristics in the CSF of human prion disease patients are associated with disease subtypes and rate of decline as defined by disease duration. |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Greece | 1 | 1% |
| Luxembourg | 1 | 1% |
| Unknown | 67 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 23% |
| Student > Ph. D. Student | 11 | 16% |
| Student > Doctoral Student | 6 | 9% |
| Professor | 6 | 9% |
| Student > Postgraduate | 6 | 9% |
| Other | 8 | 12% |
| Unknown | 16 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 16% |
| Biochemistry, Genetics and Molecular Biology | 11 | 16% |
| Medicine and Dentistry | 8 | 12% |
| Neuroscience | 5 | 7% |
| Immunology and Microbiology | 3 | 4% |
| Other | 13 | 19% |
| Unknown | 18 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2022.
All research outputs
#3,771,957
of 23,221,875 outputs
Outputs from Molecular Neurobiology
#825
of 3,518 outputs
Outputs of similar age
#37,338
of 228,305 outputs
Outputs of similar age from Molecular Neurobiology
#12
of 49 outputs
Altmetric has tracked 23,221,875 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,518 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,305 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.