| Title |
Guanidine Hydrochloride Inhibits Hsp104 Activity In Vivo: A Possible Explanation for Its Effect in Curing Yeast Prions
|
|---|---|
| Published in |
Current Microbiology, July 2001
|
| DOI | 10.1007/s002840010251 |
| Pubmed ID | |
| Authors |
Giman Jung, Daniel C. Masison |
| Abstract |
The presence of millimolar concentrations of guanidine hydrochloride (Gdn-HCl) in growth media causes efficient loss of the normally stable [PSI+] element from yeast cells. Although it has become common practice to include 5 mm Gdn-HCl in growth media to cure [PSI+] and other prions of yeast, the biochemical mechanism by which it cures is unknown. We find that 5 mm Gdn-HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance. Gdn-HCl also reduced the ability of Hsp104 to restore activity of thermally denatured luciferase in vivo. The abundance of Hsp104 was not reduced in cells grown in the presence of Gdn-HCl, ruling out negative effects on expression or stability of Hsp104. We therefore conclude that Gdn-HCl inhibits Hsp104 activity in vivo. Since replication of yeast prions is dependent on Hsp104, our results suggest that Gdn-HCl cures prions by inhibiting Hsp104 activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 62 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 29% |
| Student > Ph. D. Student | 18 | 29% |
| Student > Bachelor | 7 | 11% |
| Student > Doctoral Student | 4 | 6% |
| Professor | 4 | 6% |
| Other | 6 | 10% |
| Unknown | 5 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 45% |
| Biochemistry, Genetics and Molecular Biology | 19 | 31% |
| Immunology and Microbiology | 2 | 3% |
| Engineering | 2 | 3% |
| Physics and Astronomy | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 7 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 August 2017.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Current Microbiology
#1,543
of 2,662 outputs
Outputs of similar age
#37,384
of 40,891 outputs
Outputs of similar age from Current Microbiology
#7
of 8 outputs
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