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X Demographics
Mendeley readers
Attention Score in Context
| Title |
*Mimicking the Biochemical and Mechanical Extracellular Environment of the Endochondral Ossification Process to Enhance the In Vitro Mineralization Potential of Human Mesenchymal Stem Cells
|
|---|---|
| Published in |
Tissue Engineering: Part A, October 2017
|
| DOI | 10.1089/ten.tea.2017.0052 |
| Pubmed ID | |
| Authors |
Fiona E. Freeman, Jessica Schiavi, Meadhbh A. Brennan, Peter Owens, Pierre Layrolle, Laoise M. McNamara |
| Abstract |
Chondrogenesis and mechanical stimulation of the cartilage template, are essential for bone formation via the endochondral ossification process in vivo. Recent studies have demonstrated that in vitro regeneration strategies that mimic these aspects separately, either chondrogenesis or mechanical stimulation, can promote mineralization to a certain extent both in vitro and in vivo. However, to date no study has sought to incorporate both the formation of the cartilage template and the application of mechanical stimulation simultaneously to induce osteogenesis. In this study we test the hypothesis that mimicking both the biochemical and mechanical extracellular environment arising during endochondral ossification can enhance the in vitro mineralization potential of human Mesenchymal Stem Cells (hMSCs). hMSC cellular aggregates were cultured for 21 days under the following culture conditions; (1) Growth Medium-Hydrostatic Pressure (HP), (2) Chondrogenic Priming -HP, (3) Growth Medium +HP, (4) Chondrogenic Priming +HP. Each group was then further cultured for another 21 days in the presence of osteogenic growth factors without HP. Biochemical (DNA, sGAG, hydroxyproline, ALP, and calcium), histological (Alcian Blue, Alizarin Red), and immunohistological (Col I, II, X, BSP-2) analyses were conducted to investigate chondrogenic and osteogenic differentiation at various time points (14, 21, 35 and 42 days). Our results showed the application of HP induced chondrogenesis similar to that of chondrogenic priming but interestingly there was a reduction in hypertrophy markers (Collagen Type X) by applying HP alone versus chondrogenic priming alone. Moreover, the results showed that both chondrogenic priming and HP in tandem during the priming period, followed by culture in osteogenic medium accelerated the osteogenic potential of hMSCs. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ireland | 4 | 40% |
| Austria | 1 | 10% |
| United Kingdom | 1 | 10% |
| France | 1 | 10% |
| Unknown | 3 | 30% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 5 | 50% |
| Members of the public | 4 | 40% |
| Science communicators (journalists, bloggers, editors) | 1 | 10% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 32% |
| Researcher | 6 | 16% |
| Student > Master | 5 | 13% |
| Student > Bachelor | 2 | 5% |
| Student > Doctoral Student | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 9 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 7 | 18% |
| Engineering | 7 | 18% |
| Biochemistry, Genetics and Molecular Biology | 3 | 8% |
| Agricultural and Biological Sciences | 3 | 8% |
| Computer Science | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 15 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 January 2018.
All research outputs
#4,761,537
of 25,382,440 outputs
Outputs from Tissue Engineering: Part A
#248
of 1,842 outputs
Outputs of similar age
#78,727
of 334,091 outputs
Outputs of similar age from Tissue Engineering: Part A
#4
of 32 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,842 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 334,091 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.