Title |
Knockdown of miR-155 protects microglia against LPS-induced inflammatory injury via targeting RACK1: a novel research for intracranial infection
|
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Published in |
Journal of Inflammation, August 2017
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DOI | 10.1186/s12950-017-0162-7 |
Pubmed ID | |
Authors |
Haiyan Yin, Shuwen Song, Xudong Pan |
Abstract |
Intracranial infection, one of the complications of traumatic brain injury, is usually associated with inflammation. Several microRNAs (miRNAs), including miR-155, have been reported to be critical modulators in peripheral and central nervous system inflammation. In this study, we investigated the role of miR-155 in lipopolysaccharide (LPS)-induced inflammatory injury in mouse microglia BV2 cells. The expression level of miR-155 was significantly up-regulated after LPS stimulation in BV2 cells. LPS administration decreased BV2 cell viability, promoted apoptosis and increased the release of pro-inflammatory cytokines; while miR-155 knockdown rescued BV2 cell from LPS-induced injury. RACK1 was a directly target of miR-155. Interestingly, miR-155 knockdown did not attenuate LPS-induced inflammatory injury when RACK1 was knocked down. The mechanistic study indicated that miR-155 knockdown deactivated MAPK/NF-κB and mTOR signaling pathways under LPS-treated conditions. Knockdown of miR-155 protected mouse microglia BV2 cells from LPS-induced inflammatory injury via targeting RACK1 and deactivating MAPK/NF-κB and mTOR signaling pathways. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 25% |
Canada | 1 | 25% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 34 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 7 | 21% |
Researcher | 5 | 15% |
Student > Ph. D. Student | 4 | 12% |
Student > Doctoral Student | 3 | 9% |
Student > Bachelor | 2 | 6% |
Other | 4 | 12% |
Unknown | 9 | 26% |
Readers by discipline | Count | As % |
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Neuroscience | 7 | 21% |
Biochemistry, Genetics and Molecular Biology | 6 | 18% |
Medicine and Dentistry | 3 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Arts and Humanities | 2 | 6% |
Other | 5 | 15% |
Unknown | 9 | 26% |