| Title |
Transcriptome analysis of salinity responsiveness in contrasting genotypes of finger millet (Eleusine coracana L.) through RNA-sequencing
|
|---|---|
| Published in |
Plant Molecular Biology, May 2014
|
| DOI | 10.1007/s11103-014-0199-4 |
| Pubmed ID | |
| Authors |
Hifzur Rahman, N. Jagadeeshselvam, R. Valarmathi, B. Sachin, R. Sasikala, N. Senthil, D. Sudhakar, S. Robin, Raveendran Muthurajan |
| Abstract |
Finger millet (Eleusine coracana L.) is a hardy cereal known for its superior level of tolerance against drought, salinity, diseases and its nutritional properties. In this study, attempts were made to unravel the physiological and molecular basis of salinity tolerance in two contrasting finger millet genotypes viz., CO 12 and Trichy 1. Physiological studies revealed that the tolerant genotype Trichy 1 had lower Na(+) to K(+) ratio in leaves and shoots, higher growth rate (osmotic tolerance) and ability to accumulate higher amount of total soluble sugar in leaves under salinity stress. We sequenced the salinity responsive leaf transcriptome of contrasting finger millet genotypes using IonProton platform and generated 27.91 million reads. Mapping and annotation of finger millet transcripts against rice gene models led to the identification of salinity responsive genes and genotype specific responses. Several functional groups of genes like transporters, transcription factors, genes involved in cell signaling, osmotic homeostasis and biosynthesis of compatible solutes were found to be highly up-regulated in the tolerant Trichy 1. Salinity stress inhibited photosynthetic capacity and photosynthesis related genes in the susceptible genotype CO 12. Several genes involved in cell growth and differentiation were found to be up-regulated in both the genotypes but more specifically in tolerant genotype. Genes involved in flavonoid biosynthesis were found to be down-regulated specifically in the salinity tolerant Trichy 1. This study provides a genome-wide transcriptional analysis of two finger millet genotypes differing in their level of salinity tolerance during a gradually progressing salinity stress under greenhouse conditions. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 83 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| India | 1 | 1% |
| Unknown | 81 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 20% |
| Researcher | 17 | 20% |
| Student > Doctoral Student | 7 | 8% |
| Student > Master | 6 | 7% |
| Professor | 4 | 5% |
| Other | 10 | 12% |
| Unknown | 22 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 40 | 48% |
| Biochemistry, Genetics and Molecular Biology | 11 | 13% |
| Chemistry | 2 | 2% |
| Environmental Science | 1 | 1% |
| Social Sciences | 1 | 1% |
| Other | 3 | 4% |
| Unknown | 25 | 30% |
Attention Score in Context
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#20,230,558
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#2,622
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Outputs of similar age from Plant Molecular Biology
#4
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