| Title |
APols-Aided Protein Precipitation: A Rapid Method for Concentrating Proteins for Proteomic Analysis
|
|---|---|
| Published in |
The Journal of Membrane Biology, May 2014
|
| DOI | 10.1007/s00232-014-9668-6 |
| Pubmed ID | |
| Authors |
Zhibin Ning, Brett Hawley, Deeptee Seebun, Daniel Figeys |
| Abstract |
Amphipols (APols) are a newly designed and milder class of detergent. They have been used primarily in protein structure analysis for membrane protein trapping and stabilization. We have recently demonstrated that APols can be used as an alternative detergent for proteome extraction and digestion, to achieve a "One-stop" single-tube workflow for proteomics. In this workflow, APols are removed by precipitation after protein digestion without depleting the digested peptides. Here, we took further advantage of this precipitation characteristic of APols to concentrate proteins from diluted samples. In contrast with tryptic peptides, a decrease in pH leads to the unbiased co-precipitation of APols with proteins, including globular hydrophilic proteins. We demonstrated that this precipitation is a combined effect of acid precipitation and the APols' protein interactions. Also, we have been able to demonstrate that APols-aided protein precipitation works well on diluted samples, such as secretome sample, and provides a rapid method for protein concentration. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 3% |
| Canada | 1 | 3% |
| Unknown | 35 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 27% |
| Student > Bachelor | 9 | 24% |
| Student > Ph. D. Student | 5 | 14% |
| Student > Postgraduate | 4 | 11% |
| Student > Master | 4 | 11% |
| Other | 4 | 11% |
| Unknown | 1 | 3% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 38% |
| Agricultural and Biological Sciences | 10 | 27% |
| Computer Science | 2 | 5% |
| Engineering | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 2 | 5% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 June 2015.
All research outputs
#16,720,137
of 25,371,288 outputs
Outputs from The Journal of Membrane Biology
#616
of 820 outputs
Outputs of similar age
#138,979
of 241,369 outputs
Outputs of similar age from The Journal of Membrane Biology
#6
of 9 outputs
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