Title |
Single-Cell Gene Expression Profiles Define Self-Renewing, Pluripotent, and Lineage Primed States of Human Pluripotent Stem Cells
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Published in |
Stem Cell Reports, May 2014
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DOI | 10.1016/j.stemcr.2014.04.014 |
Pubmed ID | |
Authors |
Shelley R. Hough, Matthew Thornton, Elizabeth Mason, Jessica C. Mar, Christine A. Wells, Martin F. Pera |
Abstract |
Pluripotent stem cells display significant heterogeneity in gene expression, but whether this diversity is an inherent feature of the pluripotent state remains unknown. Single-cell gene expression analysis in cell subsets defined by surface antigen expression revealed that human embryonic stem cell cultures exist as a continuum of cell states, even under defined conditions that drive self-renewal. The majority of the population expressed canonical pluripotency transcription factors and could differentiate into derivatives of all three germ layers. A minority subpopulation of cells displayed high self-renewal capacity, consistently high transcripts for all pluripotency-related genes studied, and no lineage priming. This subpopulation was characterized by its expression of a particular set of intercellular signaling molecules whose genes shared common regulatory features. Our data support a model of an inherently metastable self-renewing population that gives rise to a continuum of intermediate pluripotent states, which ultimately become primed for lineage specification. |
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Unknown | 4 | 40% |
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Mendeley readers
Geographical breakdown
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Demographic breakdown
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Researcher | 43 | 25% |
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Professor > Associate Professor | 9 | 5% |
Professor | 8 | 5% |
Other | 28 | 17% |
Unknown | 20 | 12% |
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Medicine and Dentistry | 3 | 2% |
Other | 12 | 7% |
Unknown | 22 | 13% |