| Title |
Applications of TALENs and CRISPR/Cas9 in Human Cells and Their Potentials for Gene Therapy
|
|---|---|
| Published in |
Molecular Biotechnology, May 2014
|
| DOI | 10.1007/s12033-014-9771-z |
| Pubmed ID | |
| Authors |
Jingwen Niu, Bin Zhang, Hu Chen |
| Abstract |
The newly developed TALENs and emerging CRISPR/Cas9 have spurred interests in the field of genome engineering because of their ease of customization and high-efficient site-specific cleavages. Although these novel technologies have been successfully used in many types of cells, it is of great importance to apply them in human-derived cells to further observe and evaluate their clinical potentials in gene therapy. Here, we review the working mechanism of TALEN and CRISPR/Cas9, their effectiveness and specificity in human cells, and current methods to enhance efficiency and reduce off-target effects. Besides, CCR5 gene was chosen as a target example to illustrate their clinical potentials. Finally, some questions are raised for future research and for researchers to consider when making a proper choice bases on different purposes. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 50% |
| Science communicators (journalists, bloggers, editors) | 2 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 156 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 1% |
| United States | 2 | 1% |
| United Kingdom | 1 | <1% |
| Unknown | 151 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 34 | 22% |
| Student > Bachelor | 28 | 18% |
| Researcher | 27 | 17% |
| Student > Ph. D. Student | 23 | 15% |
| Other | 6 | 4% |
| Other | 18 | 12% |
| Unknown | 20 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 64 | 41% |
| Biochemistry, Genetics and Molecular Biology | 36 | 23% |
| Medicine and Dentistry | 18 | 12% |
| Neuroscience | 3 | 2% |
| Engineering | 3 | 2% |
| Other | 10 | 6% |
| Unknown | 22 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2022.
All research outputs
#3,782,636
of 23,414,653 outputs
Outputs from Molecular Biotechnology
#99
of 999 outputs
Outputs of similar age
#37,114
of 228,088 outputs
Outputs of similar age from Molecular Biotechnology
#1
of 13 outputs
Altmetric has tracked 23,414,653 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 999 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,088 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.