| Title |
Isolation of a Bacteriocin-Producing Lactococcus lactis and Application of Its Bacteriocin to Manage Spoilage Bacteria in High-Value Marine Fish Under Different Storage Temperatures
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, May 2012
|
| DOI | 10.1007/s12010-012-9701-0 |
| Pubmed ID | |
| Authors |
A. R. Sarika, A. P. Lipton, M. S. Aishwarya, R. S. Dhivya |
| Abstract |
The bacteriocins of lactic acid bacteria have considerable potential for biopreservation. The Lactococcus lactis strain PSY2 (GenBank account no. JF703669) isolated from the surface of marine perch Perca flavescens produced antibacterial activity against pathogenic and spoilage-causing Gram-positive and Gram-negative bacteria viz. Arthrobacter sp., Acinetobacter sp., Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa and Staphylococcus aureus and possessed broad inhibitory spectrum. The biopreservative efficacy of the bacteriocin PSY2 was evaluated using fillets of reef cod, Epinephelus diacanthus. The fillets (10 g) were sprayed with 2.0 ml of 1,600 AU/ml bacteriocin, wrapped and kept under different storage temperatures viz., 4, 0 and -18 °C. The biopreservative extended the shelf-life of fillets stored at 4 °C to >21 days as against <14 days observed in the untreated samples. The total count of spoilage bacteria was reduced by 2.5 logarithmic units in the treated sample during the 14th day of storage as against the control. Chemical analysis revealed a significant change (P < 0.05) in the pH value, free fatty acid (as % oleic acid), total volatile base nitrogen and total methyl amine content in the treated samples. The overall acceptability in terms of sensory attributes was significantly higher in the bacteriocin-treated samples stored for 21 days at 4 °C while the untreated samples became unacceptable by the 14th day. The biopreservative gave no significant effect at -18 °C. Thus, the bacteriocin derived from L. lactis PSY2 gave increased protection against spoilage bacteria and offers an alternative for the preservation of high-value sea foods. |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Czechia | 1 | 2% |
| Unknown | 52 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 21% |
| Student > Ph. D. Student | 8 | 15% |
| Researcher | 6 | 11% |
| Student > Bachelor | 5 | 9% |
| Student > Doctoral Student | 3 | 6% |
| Other | 5 | 9% |
| Unknown | 15 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 23% |
| Immunology and Microbiology | 7 | 13% |
| Biochemistry, Genetics and Molecular Biology | 3 | 6% |
| Social Sciences | 3 | 6% |
| Veterinary Science and Veterinary Medicine | 2 | 4% |
| Other | 6 | 11% |
| Unknown | 20 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 May 2014.
All research outputs
#20,230,558
of 22,756,196 outputs
Outputs from Applied Biochemistry and Biotechnology
#2,028
of 2,498 outputs
Outputs of similar age
#148,359
of 163,592 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#25
of 28 outputs
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