| Title |
Enhanced Survival and Neurite Network Formation of Human Umbilical Cord Blood Neuronal Progenitors in Three-Dimensional Collagen Constructs
|
|---|---|
| Published in |
Journal of Molecular Neuroscience, December 2012
|
| DOI | 10.1007/s12031-012-9933-z |
| Pubmed ID | |
| Authors |
Marian M. Bercu, Hadar Arien-Zakay, Dana Stoler, Shimon Lecht, Peter I. Lelkes, Simcha Samuel, Reuven Or, Arnon Nagler, Philip Lazarovici, Uriel Elchalal |
| Abstract |
Umbilical cord blood (CB) stem cells have been proposed for cell-based therapeutic applications for diverse diseases of the CNS. We hypothesized that tissue-engineering strategies may extend the efficacy of these approaches by improving the long-term viability and function of stem cell-derived neuronal progenitors. To test our hypothesis, we explored the survival and differentiation of human CB-derived neuronal progenitors (HUCBNP) in a three-dimensional (3D) collagen construct. In contrast to two-dimensional culture conditions, the cells survived in 3D for an extended period of time of more than 2 months. Under 3D conditions, HUCBNP underwent spontaneous neuronal differentiation, which was further enhanced by treatment with neuronal conditioned medium (CM) and nerve growth factor (NGF). Neurite outgrowth, quantified by assessing the fractal dimension (D f) of the complex neuronal networks, was significantly enhanced under 3D conditions in the presence of CM/NGF, concomitant with a reduced expression of the early neuronal marker nestin (1.9-fold), and increased levels of mature neuronal markers such as MAP-2 (3.6-fold), β-tubulin (1.5-fold), and neuronal specific enolase (6.6-fold) and the appearance of the synaptic marker synaptophysin. To assess the feasibility for clinical usage, HUCBNP were also isolated from frozen CB samples and cultured under 3D conditions. The data indicate the essential complete preservation of neurotrophic (survival) and neurotropic (neurite outgrowth) properties. In conclusion, 3D culture conditions are proposed as an essential step for both maintenance of CB neuronal progenitors in vitro and for investigating specific features of neuronal differentiation towards future use in regenerative therapy. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 7% |
| Unknown | 39 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 24% |
| Student > Master | 6 | 14% |
| Researcher | 5 | 12% |
| Student > Bachelor | 5 | 12% |
| Professor | 4 | 10% |
| Other | 4 | 10% |
| Unknown | 8 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 17% |
| Medicine and Dentistry | 7 | 17% |
| Biochemistry, Genetics and Molecular Biology | 5 | 12% |
| Materials Science | 4 | 10% |
| Engineering | 3 | 7% |
| Other | 8 | 19% |
| Unknown | 8 | 19% |
Attention Score in Context
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#20,656,820
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Outputs from Journal of Molecular Neuroscience
#1,156
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Outputs of similar age from Journal of Molecular Neuroscience
#9
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