| Title |
Growth Regulator Requirement for In Vitro Embryogenic Cultures of Snowdrop (Galanthus Nivalis L.) Suitable for Germplasm Preservation
|
|---|---|
| Published in |
Biologia Futura, December 2014
|
| DOI | 10.1556/abiol.65.2014.2.5 |
| Pubmed ID | |
| Authors |
Anna Resetár, Zita Demeter, Emese Ficsor, Andrea Balázs, Ágnes Mosolygó, Éva Szőke, S. Gonda, L. Pap, G. Surányi, C. Máthé |
| Abstract |
In this study, we report on the production of bulb scale-derived tissue cultures capable of efficient shoot and plant regeneration in three genotypes of snowdrop (Galanthus nivalis L., Amaryllidaceae), a protected ornamental plant. For culture line A, high auxin and low cytokinin concentration is required for callus production and plant regeneration. The type of auxin is of key importance: α-naphthaleneacetic acid (NAA) in combination with indole-3-acetic acid (IAA) at concentrations of 2 mg L-1 or 2-10 mg L-1 NAA with 1 mg L-1 N6-benzyladenine (BA), a cytokinin on full-strength media are required for regeneration. Cultures showing regeneration were embryogenic. When lines B and C were induced and maintained with 2 mg L-1 NAA and 1 mg L-1 BA, they produced mature bulblets with shoots, without roots. Line A produced immature bulblets with shoots under the above culture condition. Amplified Fragment Length Polymorphism (AFLP) analysis showed that (i) genetic differences between line A and its bulb explants were not significant, therefore these tissue cultures are suitable for germplasm preservation, and (ii) different morphogenetic responses of lines A, B and C originated from genetic differences. Culture line A is suitable for field-growing, cultivation and germplasm preservation of G. nivalis and for the production of Amaryllidaceae alkaloids. |
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| Unknown | 1 | 100% |
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|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 33% |
| Researcher | 2 | 22% |
| Professor | 1 | 11% |
| Student > Master | 1 | 11% |
| Lecturer > Senior Lecturer | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 33% |
| Chemistry | 2 | 22% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Energy | 1 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |