| Title |
The overexpression of Hsp90B1 is associated with tumorigenesis of canine mammary glands
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, August 2017
|
| DOI | 10.1007/s11010-017-3152-4 |
| Pubmed ID | |
| Authors |
B. V. Sunil Kumar, Rabia Bhardwaj, Kanika Mahajan, Neeraj Kashyap, Ashwani Kumar, Ramneek Verma |
| Abstract |
Heat shock proteins (Hsp) are molecular chaperones that are responsible for protein folding and maintenance of cellular homeostasis. Hsp90, an important member of HSP family, has an important role in breast cancer. Glucose-regulated protein 94 (Grp94) is the endoplasmic reticulum paralog of Hsp90 encoded by Hsp90B1 gene. To test if this protein is overexpressed in dogs with mammary tumor, we estimated and compared its serum levels in healthy dogs and that of dogs with mammary tumors. Hsp90B1 mRNA expression was measured in tumorous and healthy mammary tissues (from age- and breed-matched dogs) by real-time PCR. The gene was found to be overexpressed in mammary tumors (3.586 ± 0.067 times). Further, it was heterologously expressed in a prokaryotic system as 90 kDa protein. A recombinant Grp94-based sandwich ELISA was developed to quantify serum Grp94 in dogs with mammary tumors. Based on receiver-operating characteristics' analysis, the assay was found to be 90.62% sensitive and 93.75% specific for a cutoff value of 0.35 with respect to histopathological staining in diagnosing the disease. The t test showed that serum Grp94 levels were significantly elevated (92.97 ± 3.62 ng/ml) in dogs with mammary tumors compared with healthy controls (10.30 ± 0.79 ng/ml) (p < 0.0001). These findings suggest that Grp94 might act as a potential biomarker for prognosis of canine mammary tumors and monitoring its therapy. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 21% |
| Student > Master | 4 | 12% |
| Student > Ph. D. Student | 4 | 12% |
| Other | 3 | 9% |
| Professor > Associate Professor | 3 | 9% |
| Other | 3 | 9% |
| Unknown | 10 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 8 | 24% |
| Biochemistry, Genetics and Molecular Biology | 4 | 12% |
| Agricultural and Biological Sciences | 4 | 12% |
| Medicine and Dentistry | 2 | 6% |
| Chemistry | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 10 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2020.
All research outputs
#14,077,971
of 22,997,544 outputs
Outputs from Molecular and Cellular Biochemistry
#1,217
of 2,320 outputs
Outputs of similar age
#170,351
of 318,693 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#6
of 37 outputs
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