Title |
Enhanced l-Lactic Acid Production from Biomass-Derived Xylose by a Mutant Bacillus coagulans
|
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Published in |
Applied Biochemistry and Biotechnology, May 2014
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DOI | 10.1007/s12010-014-0975-2 |
Pubmed ID | |
Authors |
Zhaojuan Zheng, Cong Cai, Ting Jiang, Mingyue Zhao, Jia Ouyang |
Abstract |
Xylose effective utilization is crucial for production of bulk chemicals from low-cost lignocellulosic substrates. In this study, an efficient L-lactate production process from xylose by a mutant Bacillus coagulans NL-CC-17 was demonstrated. The nutritional requirements for L-lactate production by B. coagulans NL-CC-17 were optimized statistically in shake flask fermentations. Corn steep liquor powder and yeast exact were identified as the most significant factors by the two-level Plackett-Burman design. Steepest ascent experiments were applied to approach the optimal region of the two factors, and a central composite design was employed to determine their optimal levels. The optimal medium was used to perform batch fermentation in a 3-l bioreactor. A maximum of 90.29 g l(-1) L-lactic acid was obtained from 100 g l(-1) xylose in 120 h. When using corn stove prehydrolysates as substrates, 23.49 g l(-1) L-lactic acid was obtained in 36 h and the yield was 83.09 %. |
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Chemical Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 8 | 35% |