Title |
Neomycin biosynthesis is regulated positively by AfsA-g and NeoR in Streptomyces fradiae CGMCC 4.7387
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Published in |
Science China Life Sciences, August 2017
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DOI | 10.1007/s11427-017-9120-8 |
Pubmed ID | |
Authors |
Xiangxi Meng, Wenzhao Wang, Zhoujie Xie, Pengwei Li, Yue Li, Zhengyan Guo, Yingjian Lu, Jie Yang, Kaile Guan, Zhaoxin Lu, Huarong Tan, Yihua Chen |
Abstract |
Neomycins are a group of aminoglycoside antibiotics with both clinical and agricultural applications. To elucidate the regulatory mechanism of neomycin biosynthesis, we completed draft genome sequencing of a neomycin producer Streptomyces fradiae CGMCC 4.7387 from marine sediments, and the neomycin biosynthesis gene cluster was identified. Inactivation of the afsA-g gene encoding a γ-butyrolactone (GBL) synthase in S. fradiae CGMCC 4.7387 resulted in a significant decrease of neomycin production. Quantitative RT-PCR analysis revealed that the transcriptional level of neoR and the aphA-neoGH operon were reduced in the afsA-g::aac(3)IV mutant. Interestingly, a conserved binding site of AdpA, a key activator in the GBL regulatory cascade, was discovered upstream of neoR, a putative regulatory gene encoding a protein with an ATPase domain and a tetratricopeptide repeat domain. When neoR was inactivated, the neomycin production was reduced about 40% in comparison with the WT strain. Quantitative RT-PCR analysis revealed that the transcriptional levels of genes in the aphA-neoGH operon were reduced clearly in the neoR::aac(3)IV mutant. Finally, the titers of neomycin were improved considerably by overexpression of afsA-g and neoR in S. fradiae CGMCC 4.7387. |
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United Kingdom | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 12 | 100% |
Demographic breakdown
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Student > Doctoral Student | 3 | 25% |
Student > Master | 2 | 17% |
Student > Ph. D. Student | 2 | 17% |
Other | 1 | 8% |
Student > Bachelor | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Environmental Science | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |