| Title |
Development of the first gene expression system for Salinicoccus strains with potential application in bioremediation of hypersaline wastewaters
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, August 2017
|
| DOI | 10.1007/s00253-017-8428-9 |
| Pubmed ID | |
| Authors |
Dahe Zhao, Haibo Yang, Junyu Chen, Feiyue Cheng, Sumit Kumar, Jing Han, Ming Li, Jian Zhou, Hua Xiang |
| Abstract |
Salinicoccus salsiraiae IM408 (=CGMCC13032) is a novel halophilic bacterium that we isolated from the saline soil of Da Gang Oilfield. It tolerates 60 g/l sodium chloride and up to 123 g/l (1.5 M) sodium acetate and has shown a potential application in bioremediation of wastewater with high salt and high chemical oxygen demand (COD). Two plasmids, pS408-1 and pS408-2, were identified in S. salsiraiae IM408, and the sequences and copy numbers of the plasmids were determined. Based on these plasmids, two shuttle vectors containing a replicon for Escherichia coli, ampicillin, and chloramphenicol resistance genes, as well as the replicon from pS408-1 or pS408-2, were constructed and named as pTCS101 and pTCS201, respectively. A suitable host strain, named S. salsiraiae PE01, was also developed from the wild-type by plasmid elimination. Using the plasmid pTCS101 as an expression vector, L-lactate dehydrogenase from Staphylococcus aureus was expressed successfully in S. salsiraiae PE01. This is the first gene expression system for the Salinicoccus genus. It has provided the potential for expression of desired proteins or for establishment of desired pathways in Salinicoccus strains, which would make these halophiles more advantageous in future biotechnological applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 19% |
| Other | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Master | 2 | 13% |
| Student > Bachelor | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 44% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Engineering | 1 | 6% |
| Unknown | 6 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2017.
All research outputs
#6,192,096
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,239
of 8,034 outputs
Outputs of similar age
#93,379
of 321,308 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#16
of 93 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 321,308 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 93 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.