Chapter title |
Generating Genetically Modified Mice: A Decision Guide
|
---|---|
Chapter number | 1 |
Book title |
Site-Specific Recombinases
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7169-5_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
Authors |
Ivo J. Huijbers |
Abstract |
The generation of a new genetically modified mouse strain is a big hurdle to take for many researchers. It is often unclear which steps and decisions have to be made prior to obtaining the desired mouse model. This review aims to help researchers by providing a decision guide that answers the essential questions that need to be asked before generating the most suitable genetically modified mouse line in the most optimal timeframe. The review includes the latest technologies in both the stem cell culture and gene editing tools, particularly CRISPR/Cas9, and provides compatibility guidelines for selecting among the different types of genetic modifications that can be introduced in the mouse genome and the various routes for introducing these modifications into the mouse germline. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 3 | 17% |
Student > Bachelor | 2 | 11% |
Student > Master | 2 | 11% |
Student > Ph. D. Student | 2 | 11% |
Researcher | 2 | 11% |
Other | 2 | 11% |
Unknown | 5 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 33% |
Agricultural and Biological Sciences | 3 | 17% |
Environmental Science | 1 | 6% |
Psychology | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 28% |