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Site-Specific Recombinases

Overview of attention for book
Cover of 'Site-Specific Recombinases'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Generating Genetically Modified Mice: A Decision Guide
  3. Altmetric Badge
    Chapter 2 Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes
  4. Altmetric Badge
    Chapter 3 Building Cre Knockin Rat Lines Using CRISPR/Cas9
  5. Altmetric Badge
    Chapter 4 Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
  6. Altmetric Badge
    Chapter 5 Use of the DICE (Dual Integrase Cassette Exchange) System
  7. Altmetric Badge
    Chapter 6 Ligand-Controlled Site-Specific Recombination in Zebrafish
  8. Altmetric Badge
    Chapter 7 Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre
  9. Altmetric Badge
    Chapter 8 Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain
  10. Altmetric Badge
    Chapter 9 Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
  11. Altmetric Badge
    Chapter 10 Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X
  12. Altmetric Badge
    Chapter 11 Nanoparticle-Mediated Recombinase Delivery into Maize
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    Chapter 12 Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
  14. Altmetric Badge
    Chapter 13 FLPing Genes On and Off in Drosophila
  15. Altmetric Badge
    Chapter 14 Imaging Neural Architecture in Brainbow Samples
  16. Altmetric Badge
    Chapter 15 Purification and In Vitro Characterization of Zinc Finger Recombinases
  17. Altmetric Badge
    Chapter 16 Preparing Mate-Paired Illumina Libraries Using Cre Recombinase
  18. Altmetric Badge
    Chapter 17 Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination
  19. Altmetric Badge
    Chapter 18 Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways
  20. Altmetric Badge
    Chapter 19 Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family
  21. Altmetric Badge
    Chapter 20 Production of Minicircle DNA Vectors Using Site-Specific Recombinases
Attention for Chapter 2: Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes
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  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

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Chapter title
Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes
Chapter number 2
Book title
Site-Specific Recombinases
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7169-5_2
Pubmed ID
28815491
Book ISBNs
978-1-4939-7167-1, 978-1-4939-7169-5
Authors

Colin E. J. Pritchard, Lona J. Kroese, Ivo J. Huijbers

Abstract

Conditional alleles in genetically modified mice allow for the deletion of a gene of interest in a target tissue when combined with a tissue-specific Cre recombinase. A conditional allele is achieved by introducing LoxP sites around a critical exon, a gene, or a cluster of genes. Previously, conditional alleles were introduced in the mouse germline by classic gene targeting in embryonic stem cells, a challenging and time-consuming procedure. Now, conditional alleles can be generated directly in fertilized mouse eggs (zygotes) using the CRISPR/Cas9 technology. This one-step generation of mice is easier in design and faster. Here, we describe our achieved success rate, the considerations in design of a conditional allele, a detailed protocol to prepare the zygote injection mix, and the screening procedure to identify the new conditional knockout mouse strain.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 28%
Researcher 3 17%
Student > Bachelor 2 11%
Student > Ph. D. Student 2 11%
Student > Doctoral Student 1 6%
Other 3 17%
Unknown 2 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 39%
Biochemistry, Genetics and Molecular Biology 5 28%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Psychology 1 6%
Medicine and Dentistry 1 6%
Other 0 0%
Unknown 3 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 August 2017.
All research outputs
#14,951,544
of 22,997,544 outputs
Outputs from Methods in molecular biology
#4,722
of 13,151 outputs
Outputs of similar age
#243,378
of 421,196 outputs
Outputs of similar age from Methods in molecular biology
#416
of 1,074 outputs
Altmetric has tracked 22,997,544 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,196 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.

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