| Title |
Use of hairy roots extracts for 2,4-DCP removal and toxicity evaluation by Lactuca sativa test
|
|---|---|
| Published in |
Environmental Science and Pollution Research, October 2013
|
| DOI | 10.1007/s11356-013-2172-1 |
| Pubmed ID | |
| Authors |
Vanina A. Angelini, Elizabeth Agostini, María I. Medina, Paola S. González |
| Abstract |
2,4-Dichlorophenol (2,4-DCP) is widely distributed in wastewaters discharged from several industries, and it is considered as a priority pollutant due to its high toxicity. In this study, the use of different peroxidase extracts for 2,4-DCP removal from aqueous solutions was investigated. Tobacco hairy roots (HRs), wild-type (WT), and double-transgenic (DT) for tomato basic peroxidases (TPX1 and TPX2) were used to obtain different peroxidase extracts: total peroxidases (TPx), soluble peroxidases (SPx), and peroxidases ionically bound to the cell wall (IBPx). All extracts derived from DT HRs exhibited higher peroxidase activity than those obtained from WT HRs. TPx and IBPx DT extracts showed the highest catalytic efficiency values. The optimal conditions for 2,4-DCP oxidation were pH 6.5, H2O2 0.5 mM, and 200 U mL(-1) of enzyme, for all extracts analyzed. Although both TPx extracts were able to oxidize different 2,4-DCP concentrations, the removal efficiency was higher for TPx DT. Polyethylene glycol addition slightly improved 2,4-DCP removal efficiency, and it showed some protective effect on TPx WT after 2,4-DCP oxidation. In addition, using Lactuca sativa test, a reduction of the toxicity of post removal solutions was observed, for both TPx extracts. The results demonstrate that TPx extracts from both tobacco HRs appear to be promising candidate for future applications in removing 2,4-DCP from wastewaters. This is particularly true considering that these peroxidase sources are associated with low costs and are readily available. However, TPx DT has increased peroxidase activity, catalytic efficiency, and higher removal efficiency than TPx WT, probably due to the expression of TPX1 and TPX2 isoenzymes. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 20% |
| Student > Ph. D. Student | 3 | 15% |
| Student > Bachelor | 2 | 10% |
| Professor | 2 | 10% |
| Student > Doctoral Student | 2 | 10% |
| Other | 4 | 20% |
| Unknown | 3 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 20% |
| Environmental Science | 3 | 15% |
| Chemical Engineering | 2 | 10% |
| Medicine and Dentistry | 2 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Other | 4 | 20% |
| Unknown | 4 | 20% |
Attention Score in Context
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#21,420,714
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Outputs of similar age from Environmental Science and Pollution Research
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