| Title |
Advances in recombinant antibody manufacturing
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, March 2016
|
| DOI | 10.1007/s00253-016-7388-9 |
| Pubmed ID | |
| Authors |
Renate Kunert, David Reinhart |
| Abstract |
Since the first use of Chinese hamster ovary (CHO) cells for recombinant protein expression, production processes have steadily improved through numerous advances. In this review, we have highlighted several key milestones that have contributed to the success of CHO cells from the beginning of their use for monoclonal antibody (mAb) expression until today. The main factors influencing the yield of a production process are the time to accumulate a desired amount of biomass, the process duration, and the specific productivity. By comparing maximum cell densities and specific growth rates of various expression systems, we have emphasized the limiting parameters of different cellular systems and comprehensively described scientific approaches and techniques to improve host cell lines. Besides the quantitative evaluation of current systems, the quality-determining properties of a host cell line, namely post-translational modifications, were analyzed and compared to naturally occurring polyclonal immunoglobulin fractions from human plasma. In summary, numerous different expression systems for mAbs are available and also under scientific investigation. However, CHO cells are the most frequently investigated cell lines and remain the workhorse for mAb production until today. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,035 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | <1% |
| United States | 1 | <1% |
| Austria | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 1030 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 168 | 16% |
| Student > Ph. D. Student | 163 | 16% |
| Student > Master | 157 | 15% |
| Researcher | 134 | 13% |
| Student > Doctoral Student | 46 | 4% |
| Other | 96 | 9% |
| Unknown | 271 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 307 | 30% |
| Agricultural and Biological Sciences | 139 | 13% |
| Engineering | 79 | 8% |
| Chemical Engineering | 60 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 50 | 5% |
| Other | 127 | 12% |
| Unknown | 273 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 25. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2024.
All research outputs
#1,534,385
of 26,017,215 outputs
Outputs from Applied Microbiology and Biotechnology
#92
of 8,484 outputs
Outputs of similar age
#24,799
of 316,642 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#3
of 123 outputs
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So far Altmetric has tracked 8,484 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 98% of its peers.
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We're also able to compare this research output to 123 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.