Title |
An insecticidal toxin from Nephila clavata spider venom
|
---|---|
Published in |
Amino Acids, May 2017
|
DOI | 10.1007/s00726-017-2425-2 |
Pubmed ID | |
Authors |
Lin Jin, Mingqian Fang, Mengrou Chen, Chunling Zhou, Rose Ombati, Md Abdul Hakim, Guoxiang Mo, Ren Lai, Xiuwen Yan, Yumin Wang, Shilong Yang |
Abstract |
Spiders are the most successful insect predators given that they use their venom containing insecticidal peptides as biochemical weapons for preying. Due to the high specificity and potency of peptidic toxins, discoveries of insecticidal toxins from spider venom have provided an opportunity to obtain natural compounds for agricultural applications without affecting human health. In this study, a novel insecticidal toxin (μ-NPTX-Nc1a) was identified and characterized from the venom of Nephila clavata. Its primary sequence is GCNPDCTGIQCGWPRCPGGQNPVMDKCVSCCPFCPPKSAQG which was determined by automated Edman degradation, cDNA cloning, and MS/MS analysis. BLAST search indicated that Nc1a shows no similarity with known peptides or proteins, indicating that Nc1a belongs to a novel family of insecticidal peptide. Nc1a displayed inhibitory effects on NaV and KV channels in cockroach dorsal unpaired median neurons. The median lethal dose (LD50) of Nc1a on cockroach was 573 ng/g. Herein, a study that identifies a novel insecticidal toxin, which can be a potential candidate and/or template for the development of bioinsecticides, is presented. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 14% |
Other | 3 | 11% |
Student > Master | 3 | 11% |
Student > Bachelor | 2 | 7% |
Student > Postgraduate | 2 | 7% |
Other | 5 | 18% |
Unknown | 9 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 21% |
Biochemistry, Genetics and Molecular Biology | 3 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 11% |
Materials Science | 2 | 7% |
Medicine and Dentistry | 1 | 4% |
Other | 3 | 11% |
Unknown | 10 | 36% |