| Title |
Syncytin-1 in differentiating human myoblasts: relationship to caveolin-3 and myogenin
|
|---|---|
| Published in |
Cell and Tissue Research, June 2014
|
| DOI | 10.1007/s00441-014-1930-9 |
| Pubmed ID | |
| Authors |
Bolette Bjerregard, Iwona Ziomkiewicz, Alexander Schulz, Lars-Inge Larsson |
| Abstract |
Myoblasts fuse to form myotubes, which mature into skeletal muscle fibres. Recent studies indicate that an endogenous retroviral fusion gene, syncytin-1, is important for myoblast fusions in man. We have now expanded these data by examining the immunolocalization of syncytin in human myoblasts induced to fuse. Additionally, we have compared the localization of syncytin with the localization of caveolin-3 and of myogenin, which are also involved in myoblast fusion and maturation. Syncytin was localized to areas of the cell membrane and to filopodial structures connecting myoblasts to each other and to myotubes. Weaker staining was present over intracellular vesicles and tubules. Caveolin-3 was detected in the sarcolemma and in vesicles and tubules in a subset of myoblasts and myotubes. The strongest staining occurred in multinucleated myotubes. Wide-field fluorescence microscopy indicated a partial colocalization of syncytin and caveolin-3 in a subset of myoblasts. Super-resolution microscopy showed such colocalization to occur in the sarcolemma. Myogenin was restricted to nuclei of myoblasts and myotubes and the strongest staining occurred in multinucleated myotubes. Syncytin staining was observed in both myogenin-positive and myogenin-negative cells. Antisense treatment downmodulated syncytin-1 expression and inhibited myoblast cell fusions. Importantly, syncytin-1 antisense significantly decreased the frequency of multinucleated myotubes demonstrating that the treatment inhibited secondary myoblast fusions. Thus, syncytin is involved in human myoblast fusions and is localized in areas of contact between fusing cells. Moreover, syncytin and caveolin-3 might interact at the level of the sarcolemma. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Norway | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 28 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 17% |
| Professor > Associate Professor | 4 | 14% |
| Student > Master | 3 | 10% |
| Researcher | 2 | 7% |
| Student > Doctoral Student | 1 | 3% |
| Other | 4 | 14% |
| Unknown | 10 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 24% |
| Biochemistry, Genetics and Molecular Biology | 5 | 17% |
| Immunology and Microbiology | 3 | 10% |
| Medicine and Dentistry | 2 | 7% |
| Design | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 11 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 April 2015.
All research outputs
#14,075,458
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#1,303
of 2,279 outputs
Outputs of similar age
#114,126
of 230,725 outputs
Outputs of similar age from Cell and Tissue Research
#17
of 39 outputs
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