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Extracellular Vesicles

Overview of attention for book
Cover of 'Extracellular Vesicles'

Table of Contents

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    Book Overview
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    Chapter 1 Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine
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    Chapter 2 Red Blood Cells: A Source of Extracellular Vesicles
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    Chapter 3 Isolation of Extracellular Vesicles by Ultracentrifugation
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    Chapter 4 Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles
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    Chapter 5 Paper-Based for Isolation of Extracellular Vesicles
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    Chapter 6 Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis
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    Chapter 7 Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads
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    Chapter 8 Polymer-Based Purification of Extracellular Vesicles
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    Chapter 9 Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification
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    Chapter 10 Purification Protocols for Extracellular Vesicles
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    Chapter 11 Characterization of Extracellular Vesicles by Surface Plasmon Resonance
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    Chapter 12 Extracellular Vesicle Isolation and Analysis by Western Blotting
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    Chapter 13 Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis
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    Chapter 14 Characterization of Extracellular Vesicles by Flow Cytometry
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    Chapter 15 Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)
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    Chapter 16 Multi-Surface Antigen Staining of Larger Extracellular Vesicles
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    Chapter 17 Microcapillary Chip-Based Extracellular Vesicle Profiling System
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    Chapter 18 Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy
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    Chapter 19 Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy
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    Chapter 20 In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase
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    Chapter 21 Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters
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    Chapter 22 Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples
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    Chapter 23 Extracellular Vesicle miRNA Detection Using Molecular Beacons
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    Chapter 24 Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling
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    Chapter 25 An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles
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    Chapter 26 Protocol for Exosome Isolation from Small Volume of Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits
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    Chapter 27 Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation
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    Chapter 28 Isolation of Extracellular Vesicles from Breast Milk
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    Chapter 29 An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis
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    Chapter 30 Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles
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    Chapter 31 Production and Characterization of Extracellular Vesicles in Malaria
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    Chapter 32 Isolation of Extracellular Vesicles from Stem Cells
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    Chapter 33 The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model
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    Chapter 34 Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine
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    Chapter 35 Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles
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    Chapter 36 Erratum to: An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellular Vesicles
Attention for Chapter 32: Isolation of Extracellular Vesicles from Stem Cells
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Chapter title
Isolation of Extracellular Vesicles from Stem Cells
Chapter number 32
Book title
Extracellular Vesicles
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7253-1_32
Pubmed ID
28828674
Book ISBNs
978-1-4939-7251-7, 978-1-4939-7253-1
Authors

Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal L. Weintraub, Yaoliang Tang, Chen, Zixin, Li, Yongjun, Yu, Hong, Shen, Yan, Ju, Chengwei, Ma, Genshan, Liu, Yutao, Kim, Il-man, Weintraub, Neal L., Tang, Yaoliang

Abstract

Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia-reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 22%
Other 2 9%
Student > Bachelor 2 9%
Professor 2 9%
Student > Doctoral Student 1 4%
Other 2 9%
Unknown 9 39%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 17%
Medicine and Dentistry 3 13%
Engineering 2 9%
Neuroscience 2 9%
Physics and Astronomy 1 4%
Other 0 0%
Unknown 11 48%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2017.
All research outputs
#14,227,684
of 22,914,829 outputs
Outputs from Methods in molecular biology
#4,153
of 13,131 outputs
Outputs of similar age
#227,780
of 420,479 outputs
Outputs of similar age from Methods in molecular biology
#364
of 1,074 outputs
Altmetric has tracked 22,914,829 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,479 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.

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