| Chapter title |
Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles
|
|---|---|
| Chapter number | 30 |
| Book title |
Extracellular Vesicles
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7253-1_30 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7251-7, 978-1-4939-7253-1
|
| Authors |
Chris Wang, Austin Wang, Fang Wei, David T. W. Wong, Michael Tu, David T.W. Wong |
| Abstract |
In order to more fully elucidate the biogenesis of exosomes, a type of extracellular vesicles (EVs) and understand the role of EVs in disease processes, it is necessary to develop methods to capture EVs and induce the unloading of viable cargo. Traditionally, ultracentrifugation followed by chemical based EV lysis techniques is used to isolate these extracellular vesicles and release their internal content. Here, we describe a novel technique for capturing and releasing exosomal content through magnetic bead-based EV extraction coupled with electric field induced release and measurement (EFIRM). The usage of low-voltage electric fields allows the EV to be lysed without chemical treatment, and surface immobilized probes can allow for the rapid capture of the content of lysed EVs. EFIRM as an integrated EV lysing and analysis system offers great potential for the investigation of EVs in clinical and basic science contexts. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 42% |
| Researcher | 2 | 17% |
| Professor | 2 | 17% |
| Student > Doctoral Student | 1 | 8% |
| Other | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 25% |
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Arts and Humanities | 1 | 8% |
| Chemistry | 1 | 8% |
| Engineering | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |