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Animal Endo-SiRNAs

Overview of attention for book
Cover of 'Animal Endo-SiRNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Targeted Small Noncoding RNA-Directed Gene Activation in Human Cells
  3. Altmetric Badge
    Chapter 2 Isolation of Chromatoid Bodies from Mouse Testis as a Rich Source of Short RNAs
  4. Altmetric Badge
    Chapter 3 Generation of Endo-siRNAs in Xenopus laevis Oocytes.
  5. Altmetric Badge
    Chapter 4 Analysis of Endo-siRNAs in Drosophila.
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    Chapter 5 Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster.
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    Chapter 6 Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans.
  8. Altmetric Badge
    Chapter 7 Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs.
  9. Altmetric Badge
    Chapter 8 Extraction and Nonradioactive Detection of Small RNA Molecules
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    Chapter 9 p19-Mediated Enrichment and Detection of siRNAs.
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    Chapter 10 Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA
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    Chapter 11 Enhanced Detection of Small RNAs Using a Nonradioactive Approach
  13. Altmetric Badge
    Chapter 12 Computing siRNA and piRNA Overlap Signatures.
  14. Altmetric Badge
    Chapter 13 Isolation of Small Interfering RNAs Using Viral Suppressors of RNA Interference
  15. Altmetric Badge
    Chapter 14 Animal Endo-SiRNAs
  16. Altmetric Badge
    Chapter 15 Differential DNA Methylation Patterns in Endo-siRNAs Mediated Silencing of LINE-1 Retrotransposons
Attention for Chapter 13: Isolation of Small Interfering RNAs Using Viral Suppressors of RNA Interference
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  • Above-average Attention Score compared to outputs of the same age and source (51st percentile)

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Chapter title
Isolation of Small Interfering RNAs Using Viral Suppressors of RNA Interference
Chapter number 13
Book title
Animal Endo-SiRNAs
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-0931-5_13
Pubmed ID
24920367
Book ISBNs
978-1-4939-0930-8, 978-1-4939-0931-5
Authors

Marius van den Beek, Christophe Antoniewski, Clément Carré

Editors

Andreas Werner

Abstract

The tombusvirus P19 VSR (viral suppressor of RNA interference) binds siRNAs with high affinity, whereas the Flockhouse Virus (FHV) B2 VSR binds both long double-stranded RNA (dsRNA) and small interfering RNAs (siRNAs). Both VSRs are small proteins and function in plant and animal cells. Fusing a Nuclear Localization Signal (NLS) to the N-terminus shifts the localization of the VSR from cytoplasmic to nuclear, allowing researchers to specifically probe the subcellular distribution of siRNAs, and to investigate the function of nuclear and cytoplasmic siRNAs. This chapter provides a detailed protocol for the immunoprecipitation of siRNAs bound to epitope-tagged VSR and subsequent analysis by 3'-end-labeling using cytidine-3',5'-bis phosphate ([5'-(32)P]pCp) and northern blotting.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 44%
Student > Bachelor 2 22%
Student > Ph. D. Student 1 11%
Professor 1 11%
Student > Master 1 11%
Other 1 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 56%
Biochemistry, Genetics and Molecular Biology 2 22%
Arts and Humanities 1 11%
Immunology and Microbiology 1 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2014.
All research outputs
#15,866,607
of 23,577,654 outputs
Outputs from Methods in molecular biology
#5,555
of 13,410 outputs
Outputs of similar age
#193,937
of 309,121 outputs
Outputs of similar age from Methods in molecular biology
#210
of 576 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,121 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 576 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.

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