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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Microbial Lineages in Sarcoidosis. A Metagenomic Analysis Tailored for Low–Microbial Content Samples
|
|---|---|
| Published in |
American Journal of Respiratory & Critical Care Medicine, August 2017
|
| DOI | 10.1164/rccm.201705-0891oc |
| Pubmed ID | |
| Authors |
Erik L Clarke, Abigail P Lauder, Casey E Hofstaedter, Young Hwang, Ayannah S Fitzgerald, Ize Imai, Wojciech Biernat, Bartłomiej Rękawiecki, Hanna Majewska, Anna Dubaniewicz, Leslie A Litzky, Michael D Feldman, Kyle Bittinger, Milton D Rossman, Karen C Patterson, Frederic D Bushman, Ronald G Collman |
| Abstract |
The etiology of sarcoidosis is unknown, but microbial agents are suspected as triggers. We sought to identify bacterial, fungal or viral lineages in specimens from sarcoidosis patients enriched relative to controls using metagenomic DNA sequencing. Since DNA from environmental contamination contributes disproportionately to samples with low authentic microbial content, we developed improved methods for filtering environmental contamination. We analyzed specimens from sarcoidosis subjects (n=93), non-sarcoidosis control subjects (n=72) and various environmental controls (n=150). Sarcoidosis specimens consisted of two independent sets of formalin-fixed, paraffin-embedded lymph node biopsies, bronchoalveolar lavage (BAL), Kveim reagent, and fresh granulomatous spleen from a sarcoidosis patient. All specimens were analyzed by bacterial 16S and fungal ITS rRNA gene sequencing. In addition, BAL was analyzed by shotgun sequencing of fractions enriched for viral particles, and Kveim and spleen were subjected to whole-genome shotgun sequencing. In one tissue set, fungi in the Cladosporiaceae family were enriched in sarcoidosis compared to non-sarcoidosis tissues; in the other tissue set, we detected enrichment of several bacterial lineages in sarcoidosis, but not Cladosporiaceae. BAL showed limited enrichment of Aspergillus fungi. Several microbial lineages were detected in Kveim and spleen, including Cladosporium. No microbial lineage was enriched in more than one sample type after correction for multiple comparisons. Metagenomic sequencing revealed enrichment of microbes in single types of sarcoidosis samples, but limited concordance across sample types. Statistical analysis accounting for environmental contamination was essential to avoiding false positives. |
X Demographics
The data shown below were collected from the profiles of 16 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 31% |
| Mexico | 2 | 13% |
| Colombia | 1 | 6% |
| Ireland | 1 | 6% |
| Saudi Arabia | 1 | 6% |
| Unknown | 6 | 38% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 50% |
| Scientists | 5 | 31% |
| Practitioners (doctors, other healthcare professionals) | 2 | 13% |
| Science communicators (journalists, bloggers, editors) | 1 | 6% |
Mendeley readers
The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 58 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 26% |
| Student > Ph. D. Student | 10 | 17% |
| Student > Doctoral Student | 6 | 10% |
| Other | 5 | 9% |
| Student > Master | 3 | 5% |
| Other | 4 | 7% |
| Unknown | 15 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 13 | 22% |
| Biochemistry, Genetics and Molecular Biology | 7 | 12% |
| Agricultural and Biological Sciences | 6 | 10% |
| Immunology and Microbiology | 6 | 10% |
| Chemistry | 2 | 3% |
| Other | 5 | 9% |
| Unknown | 19 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 April 2019.
All research outputs
#2,372,963
of 25,382,440 outputs
Outputs from American Journal of Respiratory & Critical Care Medicine
#2,016
of 12,494 outputs
Outputs of similar age
#43,592
of 324,143 outputs
Outputs of similar age from American Journal of Respiratory & Critical Care Medicine
#34
of 161 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,494 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.9. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 324,143 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 161 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.