Title |
The cell polarity proteins Boi1p and Boi2p stimulate vesicle fusion at the plasma membrane of yeast cells
|
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Published in |
Journal of Cell Science, January 2017
|
DOI | 10.1242/jcs.206334 |
Pubmed ID | |
Authors |
Jochen Kustermann, Yehui Wu, Lucia Rieger, Dirk Dedden, Tamara Phan, Paul Walther, Alexander Dünkler, Nils Johnsson |
Abstract |
Eukaryotic cells can direct secretion to defined regions of their plasma membrane. These regions are distinguished by an elaborate architecture of proteins and lipids that are specialized to capture and fuse post-Golgi vesicles. Here we show that the proteins Boi1p and Boi2p are important elements of this area of active exocytosis at the tip of growing yeast cells. Cells lacking Boi1p and Boi2p accumulate secretory vesicles in their bud. The essential PH domains of Boi1p and Boi2p interact with Sec1p, a protein required for SNARE complex formation and vesicle fusion. Sec1p loses its tip localization in cells depleted of Boi1p and Boi2p but can partially compensate for their loss upon overexpression. The capacity to simultaneously bind phospholipids, Sec1p, multiple subunits of the exocyst, Cdc42p, and the module for generating active Cdc42p identify Boi1p and Boi2p as essential mediators between exocytosis and polar growth. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
United States | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 9 | 26% |
Student > Bachelor | 5 | 14% |
Student > Ph. D. Student | 5 | 14% |
Student > Master | 3 | 9% |
Other | 1 | 3% |
Other | 2 | 6% |
Unknown | 10 | 29% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 17 | 49% |
Agricultural and Biological Sciences | 8 | 23% |
Unknown | 10 | 29% |