| Title |
Immunoproteomics approach for EPC1 antigenic epitope prediction of G1 and G6 strains of Echinococcus granulosus
|
|---|---|
| Published in |
Parasitology Research, June 2013
|
| DOI | 10.1007/s00436-013-3489-x |
| Pubmed ID | |
| Authors |
Fazeleh Etebar, Fatemeh Jalousian, Seyed Hossein Hosseini, Somayeh Kordafshari, Ali Najafi |
| Abstract |
It is important to establish the diagnosis of cystic echinococcosis (CE) infection and begin control management. Currently, it is difficult to make an accurate diagnosis of CE without the availability of an accurate test, which requires the use of sensitive and specific antigens. Using recombinant antigens the sensitivity and specificity of the CE serology assays could be improved considerably. Recently, a highly antigenic protein named EPC1was characterized and isolated from an Echinococcus granulosus protoscoleces. The current study was designed to assess the sequences of EPC1 isolated from different intermediate hosts of E. granulosus. In addition, identification of a highly antigenic linear B cell epitope was found within EPC1 antigen candidate. The EPC1 sequence contains coding and non-coding regions and was compared between two predominant strains (G1 and G6) in Iran. Sequence polymorphism was not found in protein coding regions, suggesting that these regions may be useful for identification of protein expression as an antigen. The average antigenic activity for the whole protein is above 1.1, and hydrophobicity below 0 indicates that it is hydrophilic. Structural analysis showed alpha helical regions in amino acids 6-25, 35-44, 52-62, and 72-78. Nine B cell epitope residues were identified out of 67 total residues. The identity of EPC1 sequence in both G1 and G6 genotypes affects the antigenic efficacy of EPC1and suggests the recombinant protein will be useful in serological assays in the regions where the two strains are prevalent. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 3 | 14% |
| Student > Ph. D. Student | 3 | 14% |
| Student > Master | 3 | 14% |
| Researcher | 3 | 14% |
| Professor > Associate Professor | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 19% |
| Veterinary Science and Veterinary Medicine | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Immunology and Microbiology | 2 | 10% |
| Nursing and Health Professions | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 9 | 43% |
Attention Score in Context
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#20,231,820
of 22,757,541 outputs
Outputs from Parasitology Research
#2,875
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#172,267
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Outputs of similar age from Parasitology Research
#33
of 52 outputs
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