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Seminal plasma proteome of electroejaculated Bos indicus bulls

Overview of attention for article published in Animal Reproduction Science, May 2014
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Title
Seminal plasma proteome of electroejaculated Bos indicus bulls
Published in
Animal Reproduction Science, May 2014
DOI 10.1016/j.anireprosci.2014.04.016
Pubmed ID
Authors

J.P.A. Rego, J.M. Crisp, A.A. Moura, A.S. Nouwens, Y. Li, B. Venus, N.J. Corbet, D.H. Corbet, B.M. Burns, G.B. Boe-Hansen, M.R. McGowan

Abstract

The present study describes the seminal plasma proteome of Bos indicus bulls. Fifty-six, 24-month old Australian Brahman sires were evaluated and subjected to electroejaculation. Seminal plasma proteins were separated by 2-D SDS-PAGE and identified by mass spectrometry. The percentage of progressively motile and morphologically normal sperm of the bulls were 70.4±2.3 and 64±3.2%, respectively. A total of 108 spots were identified in the 2-D maps, corresponding to 46 proteins. Binder of sperm proteins accounted for 55.8% of all spots detected in the maps and spermadhesins comprised the second most abundant constituents. Other proteins of the Bos indicus seminal plasma include clusterin, albumin, transferrin, metalloproteinase inhibitor 2, osteopontin, epididymal secretory protein E1, apolipoprotein A-1, heat shock 70kDa protein, glutathione peroxidase 3, cathelicidins, alpha-enolase, tripeptidyl-peptidase 1, zinc-alpha-2-glycoprotein, plasma serine protease inhibitor, beta 2-microglobulin, proteasome subunit beta type-4, actin, cathepsins, nucleobinding-1, protein S100-A9, hemoglobin subunit alpha, cadherin-1, angiogenin-1, fibrinogen alpha and beta chain, ephirin-A1, protein DJ-1, serpin A3-7, alpha-2-macroglobulin, annexin A1, complement factor B, polymeric immunoglobulin receptor, seminal ribonuclease, ribonuclease-4, prostaglandin-H2 d-isomerase, platelet-activating factor acetylhydrolase, and phosphoglycerate kinase 1. In conclusion, this work uniquely portrays the Bos indicus seminal fluid proteome, based on samples from a large set of animals representing the Brahman cattle of the tropical Northern Australia. Based on putative biochemical attributes, seminal proteins act during sperm maturation, protection, capacitation and fertilization.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Korea, Republic of 1 1%
Unknown 81 99%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 18 22%
Student > Ph. D. Student 12 15%
Student > Master 10 12%
Researcher 7 9%
Student > Postgraduate 5 6%
Other 13 16%
Unknown 17 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 38%
Veterinary Science and Veterinary Medicine 14 17%
Biochemistry, Genetics and Molecular Biology 6 7%
Mathematics 1 1%
Business, Management and Accounting 1 1%
Other 4 5%
Unknown 25 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2014.
All research outputs
#22,759,452
of 25,373,627 outputs
Outputs from Animal Reproduction Science
#825
of 1,131 outputs
Outputs of similar age
#209,339
of 241,872 outputs
Outputs of similar age from Animal Reproduction Science
#7
of 12 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,131 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 241,872 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.