| Title |
Label-free peptide aptamer based impedimetric biosensor for highly sensitive detection of TNT with a ternary assembly layer
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, August 2017
|
| DOI | 10.1007/s00216-017-0576-3 |
| Pubmed ID | |
| Authors |
Yanyan Li, Manru Zhao, Haiyan Wang |
| Abstract |
We report a label-free peptide aptamer based biosensor for highly sensitive detection of TNT which was designed with a ternary assembly layer consisting of anti-TNT peptide aptamer (peptamer), dithiothreitol (DTT), and 6-mercaptohexanol (MCH), forming Au/peptamer-DTT/MCH. A linear relationship between the change in electron transfer resistance and the logarithm of the TNT concentration from 0.44 to 18.92 pM, with a detection limit of 0.15 pM, was obtained. In comparison, the detection limit of the aptasensor with a common binary assembly layer (Au/peptamer/MCH) was 0.15 nM. The remarkable improvement in the detection limit could be ascribed to the crucial role of the ternary assembly layer, providing an OH-richer hydrophilic environment and a highly compact surface layer with minimal surface defects, reducing the non-covalent binding (physisorption) of the peptamer and non-specific adsorption of TNT onto the electrode surface, leading to high sensitivity, and which can serve as a general sensing platform for the fabrication of other biosensors. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 14% |
| Researcher | 3 | 14% |
| Student > Master | 3 | 14% |
| Student > Bachelor | 2 | 10% |
| Professor | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 7 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 24% |
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Materials Science | 2 | 10% |
| Immunology and Microbiology | 1 | 5% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 7 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2017.
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#22,764,772
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Outputs from Analytical & Bioanalytical Chemistry
#7,543
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#284,296
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#110
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