| Title |
LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR)
|
|---|---|
| Published in |
Acta Neuropathologica, November 2012
|
| DOI | 10.1007/s00401-012-1068-3 |
| Pubmed ID | |
| Authors |
Andrey Korshunov, Marina Ryzhova, David T. W. Jones, Paul A. Northcott, Peter van Sluis, Richard Volckmann, Jan Koster, Rogier Versteeg, Cynthia Cowdrey, Arie Perry, Daniel Picard, Marc Rosenblum, Felice Giangaspero, Eleonora Aronica, Ulrich Schüller, Martin Hasselblatt, V. Peter Collins, Andreas von Deimling, Peter Lichter, Annie Huang, Stefan M. Pfister, Marcel Kool |
| Abstract |
Embryonal tumor with multilayered rosettes (ETMR, previously known as ETANTR) is a highly aggressive embryonal CNS tumor, which almost exclusively affects infants and is associated with a dismal prognosis. Accurate diagnosis is of critical clinical importance because of its poor response to current treatment protocols and its distinct biology. Amplification of the miRNA cluster at 19q13.42 has been identified previously as a genetic hallmark for ETMR, but an immunohistochemistry-based assay for clinical routine diagnostics [such as INI-1 for atypical teratoid rhabdoid tumor (AT/RT)] is still lacking. In this study, we screened for an ETMR-specific marker using a gene-expression profiling dataset of more than 1,400 brain tumors and identified LIN28A as a highly specific marker for ETMR. The encoded protein binds small RNA and has been implicated in stem cell pluripotency, metabolism and tumorigenesis. Using an LIN28A specific antibody, we carried out immunohistochemical analysis of LIN28A in more than 800 childhood brain-tumor samples and confirmed its high specificity for ETMR. Strong LIN28A immunoexpression was found in all 37 ETMR samples tested, whereas focal reactivity was only present in a small (6/50) proportion of AT/RT samples. All other pediatric brain tumors were completely LIN28A-negative. In summary, we established LIN28A immunohistochemistry as a highly sensitive and specific, rapid, inexpensive diagnostic tool for routine pathological verification of ETMR. |
Mendeley readers
The data shown below were compiled from readership statistics for 103 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | <1% |
| France | 1 | <1% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| China | 1 | <1% |
| Spain | 1 | <1% |
| Unknown | 97 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 23 | 22% |
| Student > Ph. D. Student | 18 | 17% |
| Student > Master | 10 | 10% |
| Student > Bachelor | 8 | 8% |
| Student > Postgraduate | 7 | 7% |
| Other | 20 | 19% |
| Unknown | 17 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 34 | 33% |
| Biochemistry, Genetics and Molecular Biology | 17 | 17% |
| Agricultural and Biological Sciences | 16 | 16% |
| Neuroscience | 10 | 10% |
| Unspecified | 2 | 2% |
| Other | 5 | 5% |
| Unknown | 19 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 June 2014.
All research outputs
#15,302,068
of 22,757,541 outputs
Outputs from Acta Neuropathologica
#2,098
of 2,364 outputs
Outputs of similar age
#100,397
of 159,211 outputs
Outputs of similar age from Acta Neuropathologica
#13
of 13 outputs
Altmetric has tracked 22,757,541 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,364 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.4. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 159,211 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.