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X Demographics
Mendeley readers
Attention Score in Context
| Title |
CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes
|
|---|---|
| Published in |
Scientific Reports, June 2014
|
| DOI | 10.1038/srep05228 |
| Pubmed ID | |
| Authors |
Yuki Hasegawa, Dave Tang, Naoko Takahashi, Yoshihide Hayashizaki, Alistair R. R. Forrest, the FANTOM consortium, Harukazu Suzuki |
| Abstract |
Standard culture of human induced pluripotent stem cells (hiPSCs) requires basic Fibroblast Growth Factor (bFGF) to maintain the pluripotent state, whereas hiPSC more closely resemble epiblast stem cells than true naïve state ES which requires LIF to maintain pluripotency. Here we show that chemokine (C-C motif) ligand 2 (CCL2) enhances the expression of pluripotent marker genes through the phosphorylation of the signal transducer and activator of transcription 3 (STAT3) protein. Moreover, comparison of transcriptomes between hiPSCs cultured with CCL2 versus with bFGF, we found that CCL2 activates hypoxia related genes, suggesting that CCL2 enhanced pluripotency by inducing a hypoxic-like response. Further, we show that hiPSCs cultured with CCL2 can differentiate at a higher efficiency than culturing with just bFGF and we show CCL2 can be used in feeder-free conditions in the absence of LIF. Taken together, our finding indicates the novel functions of CCL2 in enhancing its pluripotency in hiPSCs. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 17% |
| France | 1 | 17% |
| Japan | 1 | 17% |
| Unknown | 3 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 67% |
| Scientists | 2 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| France | 1 | 1% |
| Brazil | 1 | 1% |
| Finland | 1 | 1% |
| China | 1 | 1% |
| Japan | 1 | 1% |
| Unknown | 78 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 20 | 24% |
| Student > Ph. D. Student | 14 | 17% |
| Student > Master | 10 | 12% |
| Student > Bachelor | 9 | 11% |
| Professor | 8 | 10% |
| Other | 17 | 20% |
| Unknown | 6 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 35 | 42% |
| Biochemistry, Genetics and Molecular Biology | 20 | 24% |
| Medicine and Dentistry | 11 | 13% |
| Nursing and Health Professions | 1 | 1% |
| Business, Management and Accounting | 1 | 1% |
| Other | 8 | 10% |
| Unknown | 8 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 65. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 October 2015.
All research outputs
#551,257
of 22,757,541 outputs
Outputs from Scientific Reports
#6,182
of 122,748 outputs
Outputs of similar age
#5,592
of 228,106 outputs
Outputs of similar age from Scientific Reports
#33
of 850 outputs
Altmetric has tracked 22,757,541 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 122,748 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.2. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,106 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 850 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.