| Title |
HIV-1 Tat C phosphorylates VE-cadherin complex and increases human brain microvascular endothelial cell permeability
|
|---|---|
| Published in |
BMC Neuroscience, June 2014
|
| DOI | 10.1186/1471-2202-15-80 |
| Pubmed ID | |
| Authors |
Ritu Mishra, Sunit Kumar Singh |
| Abstract |
Human brain microvascular endothelial cells (hBMVECs) are integral part of the blood brain barrier. Post-translational modifications of adherens junction proteins regulate the permeability of human brain microvascular endothelial cells. Pro-inflammatory signals can induce tyrosine phosphorylation of adherens junction proteins. The primary objective of this work is to provide a molecular model; how the HIV-1 Tat protein can compromise the BBB integrity and eventually lead to neurological consequences. We exposed hBMVECs to recombinant HIV-1 clade C Tat protein to study the effect of HIV-1 Tat C on permeability of hBMVECs. Trans-endothelial electrical resistance and fluorescent dye migration assay have been used to check the permeability of hBMVECs. DCFDA staining has been used for intracellular reactive oxygen species (ROS) detection. Western blotting has been used to study the expression levels and co-immunoprecipitation has been used to study the interactions among adherens junction proteins. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 50% |
| India | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 23% |
| Student > Master | 7 | 16% |
| Researcher | 4 | 9% |
| Student > Bachelor | 4 | 9% |
| Student > Doctoral Student | 3 | 7% |
| Other | 10 | 23% |
| Unknown | 6 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 10 | 23% |
| Agricultural and Biological Sciences | 7 | 16% |
| Neuroscience | 6 | 14% |
| Medicine and Dentistry | 6 | 14% |
| Psychology | 3 | 7% |
| Other | 4 | 9% |
| Unknown | 8 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2022.
All research outputs
#15,745,721
of 23,390,392 outputs
Outputs from BMC Neuroscience
#717
of 1,261 outputs
Outputs of similar age
#135,340
of 229,339 outputs
Outputs of similar age from BMC Neuroscience
#18
of 34 outputs
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So far Altmetric has tracked 1,261 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
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We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.